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Citation: LIU Ke, LIU Hui-Hong, WANG Qi-Hui. An Organic Peroxide Biosensor Based on Horseradish Peroxidase and Thionine Entrapped by Carrageenan Hydrogel[J]. Chinese Journal of Applied Chemistry, ;2008, 25(4): 409-413. shu

An Organic Peroxide Biosensor Based on Horseradish Peroxidase and Thionine Entrapped by Carrageenan Hydrogel

LIU Ke ^a ,
LIU Hui-Hong ^a,b,, ,
WANG Qi-Hui ^b

1.
a College of Chemistry, Central China Normal University, Wuhan 430079;
2.
b Department of Chemistry & Biology, Xiangfan University, Xiangfan 441053

Corresponding author: LIU Hui-Hong,
Received Date: 6 April 2007
Available Online: 6 July 2007
Fund Project: 湖北省自然科学基金项目(2005ABA007) (2005ABA007)湖北省教育厅科技项目(T20030718,G200525001)资助 (T20030718,G200525001)

Horseradish peroxidase and thionine were immobilized on glassy carbon electrode surface by carrabeenan hydrogel for fabricating a peroxide biosensor.The well-defined redox peaks with the cathodic and anodic peak potentials located at about -0.264 V and -0.176 V were observed in the cyclic voltammogram for the entrapped thionone.The peak separation of 88mV and the ratio (about 1) of cathodic and anodic peak currents suggest a quasi-reversible electrochemical reaction.The mechanism of redox process coupled with proton transfer is proposed.Thionine is a good electron mediator in the reaction.Hydrogen peroxide,Cumene hydroperoxide,t-butanone peroxide and 2-butanone peroxide were reduced catalytically by horseradish peroxidase.The biosensor shows fast response,high sensitivity,good stability and reproducibility.
- carageenan,
- horseradish peroxidase,
- thionine,
- peroxides
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