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Citation: ZHANG Yu-Qin, ZHANG You-Yu, YE Min, TAN Ping, ZHAO Meng-Jiao, YAO Shou-Zhuo. Interaction of L-Cysteine-capped-ZnS Nanoparticles with Bovine Hemoglobin by using UV-Vis, Fluorenscence and FTIR Spectroscopy[J]. Chinese Journal of Applied Chemistry, ;2008, 25(9): 1011-1016. shu

Interaction of L-Cysteine-capped-ZnS Nanoparticles with Bovine Hemoglobin by using UV-Vis, Fluorenscence and FTIR Spectroscopy

1.
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081

Corresponding author: ZHANG You-Yu,
Received Date: 24 August 2007
Available Online: 29 December 2007
Fund Project: 国家自然科学基金(20675030) (20675030)教育部重点项目(207076) (207076)湖南省教育厅、科技厅重点项目(06A035,060223)资助 (06A035,060223)

ZnS nanoparticles were synthesized and characterized.The synthetic and modified conditions of ZnS nanoparticles,such as pH value,reaction temperature,the molar ratio of Zn(Ac)₂,Na₂S and the stabilizing reagent(EDTA) and the concentration of L-cysteine,were optimized.Under the best conditions of synthesis and modification,functionalized ZnS nanoparticles with sphere shape and a diameter of 150 nm were obtained.UV-Vis,fluorescence and attenuated total reflectance Fourier transform infrared(ATR-FTIR) spectroscopies were used to study the interaction of ZnS nanoparticles modified by L-cysteine and Bovine hemoglobin.The results indicate that the fluorescence of functionalized ZnS nanoparticles was significantly quenched by Bovine hemoglobin.The quenching constant calculated according to Stern-Volmer equation was 1.12×10⁶ L/mol.It is inferred from this result that the effect of Bovine hemoglobin on the fluorescence of functionalized ZnS nanoparticles was a static quenching and strong interaction taking place between Bovine hemoglobin molecules and functionalized ZnS nanoparticles.The strong interaction significantly affected the second structure of BHb molecules,which was confirmed by ATR-FTIR observations.
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