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Citation: ZHANG Chi, SONG Xi-Jina, WANG Jie, WEN Jing. Stability and Fluorescence Spectra of Lipase Labeled with FITC[J]. Chinese Journal of Applied Chemistry, ;2008, 25(12): 1381-1384. shu

Stability and Fluorescence Spectra of Lipase Labeled with FITC

ZHANG Chi ^a ,
SONG Xi-Jina ^a,, ,
WANG Jie ^b ,
WEN Jing ^a

1.
a Department of Chemical & Biochemical Engineering, Zhejiang University, Hangzhou 310027;
2.
b Department of Chemistry, Zhejiang University, Hangzhou 310027

Corresponding author: SONG Xi-Jina,
Received Date: 3 November 2007
Available Online: 24 February 2008
Fund Project: 国家自然科学基金(20276067)资助项目 (20276067)

FITC(fluorescein isothiocyanate) that can be linked to lipase as a fluorescence probe was used to study the relationships among the activity of lipase,conformation,and fluorescence spectrum.The changes of activity and fluorescence spectrum of the labeled and unlabeled lipase were examined under various temperatures,pH and different concentrations of guanidine hydrochloride.The results demonstrated that compared with the unlabeled lipase,the thermal stability of the labeled lipase was improved,and it was completely inactive at 70℃.The stability of the labelled lipase at different pH was also increased.The activity of the labeled lipase would decrease under different concentrations of guanidine hydrochloride,and fluorescence spectrum of the labeled lipase could reflect the change of the activity.
- lipase,
- FITC(fluorescein isothiocyanat),
- fluorescence spectrum,
- stability
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