Citation: Yin-Hui Li, Jin-Feng Yang, Chang-Hui Liu, Ji-Shan Li, Rong-Hua Yang. Colorimetric and fluorescent detection of biological thiols in aqueous solution[J]. Chinese Chemical Letters, ;2013, 24(2): 96-98. shu

Colorimetric and fluorescent detection of biological thiols in aqueous solution

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a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
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b Anesthesia Department, Affilicated Cancer Hospital, Xiangya School of Medicine, Central South University, Changsha 410013, China

Corresponding author: Rong-Hua Yang,
Received Date: 10 December 2012
Available Online: 10 January 2013
Fund Project: This work was supported by the National Key Natural Science Foundation of China (No. 21135001) (No. 21135001) "973" National Key Basic Research Program (No. 2011CB911000) (No. 2011CB911000) Hunan Province Key Project of Scientific & Technical Programs (No. 2010TP4013-1) (No. 2010TP4013-1)

A new colorimetric and fluorescent probe, 2-(2,4-dinitrostyryl)-1,3,3-trimethyl-3H-indolium iodide (DTI), for selective and sensitive detection of biological thiols is reported. In aqueous solution at physiological pH 7.4, biological thiols react with DTI via Michael addition to give the brownish red adduct concomitant with fluorescence emission decrease.
- Colorimetry,
- Fluorescence,
- Biological thiols,
- Michael addition
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