Citation: Cai-Jing Cui, Wen-Sheng Cai, Xue-Guang Shao. Near-infrared diffuse reflectance spectroscopy with sample spots and chemometrics for fast determination of bovine serum albumin in micro-volume samples[J]. Chinese Chemical Letters, ;2013, 24(01): 67-69. shu

Near-infrared diffuse reflectance spectroscopy with sample spots and chemometrics for fast determination of bovine serum albumin in micro-volume samples

1.
Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China

Corresponding author: Xue-Guang Shao,
Received Date: 22 October 2012
Available Online: 14 December 2012

Near-infrared diffuse reflectance spectroscopy(NIRDRS)has attracted more and more attention in analyzing the components in samples with complex matrices.However,to apply this technique to micro-analysis,there are still some obstacles to overcome such as the low sensitivity and spectral overlapping associated with this approach.A method for fast determination of bovine serum albumin (BSA)in micro-volume samples was studied using NIRDRS with sample spots and chemometric techniques.10 μL of sample spotted on a filter paper substrate was used for the spectral measurements. Quantitative analysis was obtained by partial least squares(PLS)regression with signal processing and variable selection.The results show that the correlation coefficient(R)between the predicted and the reference concentration is 0.9897 and the recoveries are in the range of 87.4%-114.4%for the validation samples in the concentration range of 0.61-8.10 mg/mL.These results suggest that the method has the potential to quickly measure proteins in micro-volume solutions.
- Near-infrared diffuse reflectance spectroscopy,
- Sample spot,
- Filter paper,
- Protein,
- Micro-volume analysis
1. [1]
  [1] M.Blanco,I.Villarroya,NIR spectroscopy:a rapid-response analytical tool,Trends Anal.Chem.21(2002)240–250.
2. [2]
  [2] J.Moros,S.Garrigues,M.de la Guardia,Vibrational spectroscopy provides a green tool for multi-component analysis,Trends Anal.Chem.29(2010)578–591.
3. [3]
  [3] M.Zhang,W.S.Cai,X.G.Shao,Wavelet unfolded partial least squares for near- infrared spectral quantitative analysis of blood and tobacco powder samples, Analyst 136(2011)4217–4221.
4. [4]
  [4] X.G.Shao,X.H.Bian,W.S.Cai,An improved boosting partial least squares method for near-infrared spectroscopic quantitative analysis,Anal.Chim.Acta 666(2010) 32–37.
5. [5]
  [5] X.G.Shao,X.H.Bian,J.J.Liu,Multivariate calibration methods in near infrared spectroscopic analysis,Anal.Methods 2(2010)1662–1666.
6. [6]
  [6] Y.N.Ni,M.H.Mei,S.Kokot,Analysis of complex,processed substances with the use of NIR spectroscopy and chemometrics:classification and prediction of properties –the potato crisps example,Chemom.Intell.Lab.Syst.105(2011)147–156.
7. [7]
  [7] N.Qi,Z.Y.Zhang,Y.H.Xiang,Locally linear embedding method for dimensionality reduction of tissue sections of endometrial carcinoma by near infrared spectros- copy,Anal.Chim.Acta 724(2012)12–19.
8. [8]
  [8] Y.P.Du,X.M.Wei,H.P.Xie,An enrichment device of silica-based monolithic material and its application to determine micro-carbaryl by NIRS,Chin.Chem. Lett.20(2009)469–472.
9. [9]
  [9] Z.X.Huang,W.Tao,J.J.Fang,Multivariate calibration of on-line enrichment near- infrared(NIR)spectra and determination of trace lead in water,Chemom.Intell. Lab.Syst.98(2009)195–200.
10. [10]
  [10] Y.Zhang,Y.Hao,W.S.Cai,Simultaneous determination of phenol and p-nitro- phenol in wastewater using near-infrared diffuse reflectance spectroscopy with adsorption preconcentration,Anal.Methods 3(2011)703–708.
11. [11]
  [11] Y.F.Yang,J.R.Tu,W.S.Cai,Feasibility for quantitative determination of deoxyr- ibonucleic acid by using near-infrared diffuse reflectance spectroscopy,Talanta 99(2012)871–874.
12. [12]
  [12] Y.Ning,J.H.Li,W.S.Cai,Simultaneous determination of heavy metal ions in water using near-infrared spectroscopy with preconcentration by nano-hydroxyapatite, Spectrochim.Acta A 96(2012)289–294.
13. [13]
  [13] K.Murayama,K.Yamada,R.Tsenkova,Near-infrared spectra of serum albumin and g-globulin and determination of their concentrations in phosphate buffer solutions by partial least squares regression,Vib.Spectrosc.18(1998)33–40.
14. [14]
  [14] A.Savitzky,M.J.E.Golay,Smoothing and Differentiation of Data by Simplified Least Squares Procedures,Anal.Chem.36(1964)1627–1639.
15. [15]
  [15] P.Geladi,D.MacDougall,H.Martens,Linearization and scatter-correction for near-infrared reflectance spectra of meat,Appl.Spectrosc.39(1985)491–500.
16. [16]
  [16] R.J.Barnes,M.S.Dhanoa,S.J.Lister,Standard normal variate transformation and de-trending of near-infrared diffuse reflectance spectra,Appl.Spectrosc.43 (1989)772–777.
17. [17]
  [17] X.G.Shao,A.K.M.Leung,F.T.Chau,Wavelet:a new trend in chemistry,Acc.Chem. Res.36(2003)276–283.
1. [1]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
2. [2]
  Wenbi Wu , Yinchu Dong , Haofan Liu , Xuebing Jiang , Li Li , Yi Zhang , Maling Gou . Modification of plasma protein for bioprinting via photopolymerization. Chinese Chemical Letters, 2024, 35(8): 109260-. doi: 10.1016/j.cclet.2023.109260
3. [3]
  Wenxiang Ma , Xinyu He , Tianyi Chen , De-Li Ma , Hongzheng Chen , Chang-Zhi Li . Near-infrared non-fused electron acceptors for efficient organic photovoltaics. Chinese Chemical Letters, 2024, 35(4): 109099-. doi: 10.1016/j.cclet.2023.109099
4. [4]
  Yang Liu , Leilei Zhang , Kaixuan Liu , Ling-Ling Wu , Hai-Yu Hu . Penicillin G acylase-responsive near-infrared fluorescent probe: Unravelling biofilm regulation and combating bacterial infections. Chinese Chemical Letters, 2024, 35(11): 109759-. doi: 10.1016/j.cclet.2024.109759
5. [5]
  Huamei Zhang , Jingjing Liu , Mingyue Li , Shida Ma , Xucong Zhou , Aixia Meng , Weina Han , Jin Zhou . Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe. Chinese Chemical Letters, 2024, 35(12): 110020-. doi: 10.1016/j.cclet.2024.110020
6. [6]
  Yixin Zhang , Ting Wang , Jixiang Zhang , Pengyu Lu , Neng Shi , Liqiang Zhang , Weiran Zhu , Nongyue He . Formation mechanism for stable system of nanoparticle/protein corona and phospholipid membrane. Chinese Chemical Letters, 2024, 35(4): 108619-. doi: 10.1016/j.cclet.2023.108619
7. [7]
  Mingqi Wang , Shixin Fa , Jiate Yu , Guoxian Zhang , Yi Yan , Qing Liu , Qiuyu Zhang . Light-controlled protein imprinted nanospheres with variable recognition specificity. Chinese Chemical Letters, 2025, 36(2): 110124-. doi: 10.1016/j.cclet.2024.110124
8. [8]
  Hui Peng , Xiao Wang , Weiguo Huang , Shuiyue Yu , Linghang Kong , Qilin Wei , Jialong Zhao , Bingsuo Zou . Efficient tunable visible and near-infrared emission in Sb³⁺/Sm³⁺-codoped Cs₂NaLuCl₆ for near-infrared light-emitting diode, triple-mode fluorescence anti-counterfeiting and information encryption. Chinese Chemical Letters, 2024, 35(11): 109462-. doi: 10.1016/j.cclet.2023.109462
9. [9]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
10. [10]
  Lei Wang , Jun-Jie Wu , Chang-Cun Yan , Wan-Ying Yang , Zong-Lu Che , Xin-Yu Xia , Xue-Dong Wang , Liang-Sheng Liao . Near-infrared organic lasers with ultra-broad emission bands by simultaneously harnessing four-level and six-level systems. Chinese Chemical Letters, 2024, 35(8): 109365-. doi: 10.1016/j.cclet.2023.109365
11. [11]
  Ying Zhao , Yin-Hang Chai , Tian Chen , Jie Zheng , Ting-Ting Li , Francisco Aznarez , Li-Long Dang , Lu-Fang Ma . Size-controlled synthesis and near-infrared photothermal response of Cp* Rh-based metalla[2]catenanes and rectangular metallamacrocycles. Chinese Chemical Letters, 2024, 35(6): 109298-. doi: 10.1016/j.cclet.2023.109298
12. [12]
  Yikun Wang , Qiaomei Chen , Shijie Liang , Dongdong Xia , Chaowei Zhao , Christopher R. McNeill , Weiwei Li . Near-infrared double-cable conjugated polymers based on alkyl linkers with tunable length for single-component organic solar cells. Chinese Chemical Letters, 2024, 35(4): 109164-. doi: 10.1016/j.cclet.2023.109164
13. [13]
  Xuan Zhu , Lin Zhou , Xiao-Yun Huang , Yan-Ling Luo , Xin Deng , Xin Yan , Yan-Juan Wang , Yan Qin , Yuan-Yuan Tang . (Benzimidazolium)2GeI4: A layered two-dimensional perovskite with dielectric switching and broadband near-infrared photoluminescence. Chinese Journal of Structural Chemistry, 2024, 43(6): 100272-100272. doi: 10.1016/j.cjsc.2024.100272
14. [14]
  Fuzheng Zhang , Chao Shi , Jiale Li , Fulin Jia , Xinyu Liu , Feiyang Li , Xinyu Bai , Qiuxia Li , Aihua Yuan , Guohua Xie . B-embedded narrowband pure near-infrared (NIR) phosphorescent iridium(Ⅲ) complexes and solution-processed OLED application. Chinese Chemical Letters, 2025, 36(1): 109596-. doi: 10.1016/j.cclet.2024.109596
15. [15]
  Haowen Shang , Yujie Yang , Bingjie Xue , Yikai Wang , Zhiyi Su , Wenlong Liu , Youzhi Wu , Xinjun Xu . Efficient solution-processed near-infrared organic light-emitting diodes with a binary-mixed electron transport layer. Chinese Chemical Letters, 2025, 36(4): 110511-. doi: 10.1016/j.cclet.2024.110511
16. [16]
  Ying Li , Long-Jie Wang , Yong-Kang Zhou , Jun Liang , Bin Xiao , Ji-Shen Zheng . An improved installation of 2-hydroxy-4-methoxybenzyl (iHmb) method for chemical protein synthesis. Chinese Chemical Letters, 2024, 35(5): 109033-. doi: 10.1016/j.cclet.2023.109033
17. [17]
  Si Ha , Jiacheng Zhu , Hua Xiang , Guoshun Luo . Hydrophobic tag tethering degrader as a promising paradigm of protein degradation: Past, present and future perspectives. Chinese Chemical Letters, 2024, 35(8): 109192-. doi: 10.1016/j.cclet.2023.109192
18. [18]
  Wenhao Wang , Siyuan Peng , Zhengwei Huang , Xin Pan . Tuning amino/hydroxyl ratios of nanovesicles to manipulate protein corona-mediated in vivo fate. Chinese Chemical Letters, 2024, 35(11): 110134-. doi: 10.1016/j.cclet.2024.110134
19. [19]
  Bo Liu , Shuaiqiang Shao , Junjie Cai , Zijian Zhang , Feng Tian , Kun Yang , Fan Li . Signal cascade amplification of streptavidin-biotin-modified immunofluorescence nanocapsules for ultrasensitive detection of glial fibrillary acidic protein. Chinese Chemical Letters, 2025, 36(3): 109814-. doi: 10.1016/j.cclet.2024.109814
20. [20]
  Xuejian Xing , Pan Zhu , E Pang , Shaojing Zhao , Yu Tang , Zheyu Hu , Quchang Ouyang , Minhuan Lan . D-A-D-structured boron-dipyrromethene with aggregation-induced enhanced phototherapeutic efficiency for near-infrared fluorescent and photoacoustic imaging-guided synergistic photodynamic and photothermal cancer therapy. Chinese Chemical Letters, 2024, 35(10): 109452-. doi: 10.1016/j.cclet.2023.109452

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(745)
HTML views(0)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142