Citation: Cai-Jing Cui, Wen-Sheng Cai, Xue-Guang Shao. Near-infrared diffuse reflectance spectroscopy with sample spots and chemometrics for fast determination of bovine serum albumin in micro-volume samples[J]. Chinese Chemical Letters, ;2013, 24(01): 67-69. shu

Near-infrared diffuse reflectance spectroscopy with sample spots and chemometrics for fast determination of bovine serum albumin in micro-volume samples

1.
Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China

Corresponding author: Xue-Guang Shao,
Received Date: 22 October 2012
Available Online: 14 December 2012

Near-infrared diffuse reflectance spectroscopy(NIRDRS)has attracted more and more attention in analyzing the components in samples with complex matrices.However,to apply this technique to micro-analysis,there are still some obstacles to overcome such as the low sensitivity and spectral overlapping associated with this approach.A method for fast determination of bovine serum albumin (BSA)in micro-volume samples was studied using NIRDRS with sample spots and chemometric techniques.10 μL of sample spotted on a filter paper substrate was used for the spectral measurements. Quantitative analysis was obtained by partial least squares(PLS)regression with signal processing and variable selection.The results show that the correlation coefficient(R)between the predicted and the reference concentration is 0.9897 and the recoveries are in the range of 87.4%-114.4%for the validation samples in the concentration range of 0.61-8.10 mg/mL.These results suggest that the method has the potential to quickly measure proteins in micro-volume solutions.
- Near-infrared diffuse reflectance spectroscopy,
- Sample spot,
- Filter paper,
- Protein,
- Micro-volume analysis
1. [1]
  [1] M.Blanco,I.Villarroya,NIR spectroscopy:a rapid-response analytical tool,Trends Anal.Chem.21(2002)240–250.
2. [2]
  [2] J.Moros,S.Garrigues,M.de la Guardia,Vibrational spectroscopy provides a green tool for multi-component analysis,Trends Anal.Chem.29(2010)578–591.
3. [3]
  [3] M.Zhang,W.S.Cai,X.G.Shao,Wavelet unfolded partial least squares for near- infrared spectral quantitative analysis of blood and tobacco powder samples, Analyst 136(2011)4217–4221.
4. [4]
  [4] X.G.Shao,X.H.Bian,W.S.Cai,An improved boosting partial least squares method for near-infrared spectroscopic quantitative analysis,Anal.Chim.Acta 666(2010) 32–37.
5. [5]
  [5] X.G.Shao,X.H.Bian,J.J.Liu,Multivariate calibration methods in near infrared spectroscopic analysis,Anal.Methods 2(2010)1662–1666.
6. [6]
  [6] Y.N.Ni,M.H.Mei,S.Kokot,Analysis of complex,processed substances with the use of NIR spectroscopy and chemometrics:classification and prediction of properties –the potato crisps example,Chemom.Intell.Lab.Syst.105(2011)147–156.
7. [7]
  [7] N.Qi,Z.Y.Zhang,Y.H.Xiang,Locally linear embedding method for dimensionality reduction of tissue sections of endometrial carcinoma by near infrared spectros- copy,Anal.Chim.Acta 724(2012)12–19.
8. [8]
  [8] Y.P.Du,X.M.Wei,H.P.Xie,An enrichment device of silica-based monolithic material and its application to determine micro-carbaryl by NIRS,Chin.Chem. Lett.20(2009)469–472.
9. [9]
  [9] Z.X.Huang,W.Tao,J.J.Fang,Multivariate calibration of on-line enrichment near- infrared(NIR)spectra and determination of trace lead in water,Chemom.Intell. Lab.Syst.98(2009)195–200.
10. [10]
  [10] Y.Zhang,Y.Hao,W.S.Cai,Simultaneous determination of phenol and p-nitro- phenol in wastewater using near-infrared diffuse reflectance spectroscopy with adsorption preconcentration,Anal.Methods 3(2011)703–708.
11. [11]
  [11] Y.F.Yang,J.R.Tu,W.S.Cai,Feasibility for quantitative determination of deoxyr- ibonucleic acid by using near-infrared diffuse reflectance spectroscopy,Talanta 99(2012)871–874.
12. [12]
  [12] Y.Ning,J.H.Li,W.S.Cai,Simultaneous determination of heavy metal ions in water using near-infrared spectroscopy with preconcentration by nano-hydroxyapatite, Spectrochim.Acta A 96(2012)289–294.
13. [13]
  [13] K.Murayama,K.Yamada,R.Tsenkova,Near-infrared spectra of serum albumin and g-globulin and determination of their concentrations in phosphate buffer solutions by partial least squares regression,Vib.Spectrosc.18(1998)33–40.
14. [14]
  [14] A.Savitzky,M.J.E.Golay,Smoothing and Differentiation of Data by Simplified Least Squares Procedures,Anal.Chem.36(1964)1627–1639.
15. [15]
  [15] P.Geladi,D.MacDougall,H.Martens,Linearization and scatter-correction for near-infrared reflectance spectra of meat,Appl.Spectrosc.39(1985)491–500.
16. [16]
  [16] R.J.Barnes,M.S.Dhanoa,S.J.Lister,Standard normal variate transformation and de-trending of near-infrared diffuse reflectance spectra,Appl.Spectrosc.43 (1989)772–777.
17. [17]
  [17] X.G.Shao,A.K.M.Leung,F.T.Chau,Wavelet:a new trend in chemistry,Acc.Chem. Res.36(2003)276–283.
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沈阳化工大学材料科学与工程学院沈阳 110142