Citation: Kai Fang, Yukun Gao, Limin Yang, Lei Jiang. Magnetic Beads-Loaded Aptamer-Modified Gold Nano Probes for Detection of Bacterial Endotoxin in Serum[J]. Chemistry, ;2021, 84(6): 563-570. shu

Magnetic Beads-Loaded Aptamer-Modified Gold Nano Probes for Detection of Bacterial Endotoxin in Serum

State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266580

Corresponding author: Limin Yang, yanglimin@upc.edu.cn Lei Jiang, leijiang@upc.edu.cn
Received Date: 17 November 2020
Accepted Date: 29 December 2020

Figures(6)

Bio-sensing detection of bacterial endotoxins (LPS) in serums is of great application value, but its development process is limited by the complexity of the substances contained in the serum. In this paper, a colorimetric sensor based on the magnetic bead-gold nanoparticles-aptamer (MB-AuNPs-APT) bioprobe was developed. LPS is captured by bioprobes to affect its peroxidase activity, this in turn affects the production which H₂O₂ catalytic TMB producing oxTMB, and finally achieve the purpose of colorimetric analysis of LPS. Meanwhile, the feasibility of LPS detection in serum is also analyzed. The results show that the detection limit using the colorimetric sensing method based on MB-AuNPs-APT bioprobes is 0.402 ng/mL and the linear range is 0.1~100 ng/mL. In the detection of LPS in 50% serum, the recovery rate of this biosensor to detect LPS ranged from 99.59 to 112.00%, demonstrated the reliability of the biological probe detecting LPS in serum.
- Biosensor,
- Lipopolysaccharide,
- Gold nanoparticles,
- Aptamer,
- Serum
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