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Citation: AN Lingling, CHANG Yingcui, DU Jiangyan. A Novel Hydrogen Peroxide Biosensor Based on the Immobilization of Catalase on Three-Dimensionally Ordered Macroporous Graphene-doped Titanium Dioxide Film[J]. Chinese Journal of Applied Chemistry, ;2013, 30(2): 171-177. doi: DOI:10.3724/SP.J.1095.2013.20148 shu

A Novel Hydrogen Peroxide Biosensor Based on the Immobilization of Catalase on Three-Dimensionally Ordered Macroporous Graphene-doped Titanium Dioxide Film

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a College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China;
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b Jiangsu Key Laboratory of Bio-functional Materials, Nanjing 210046, China;
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c Jiangsu Key Laboratory of New Power Batteries, Nanjing 210046, China

Corresponding author: DU Jiangyan,
Received Date: 12 April 2012
Available Online: 25 May 2012
Fund Project: 江苏高校优势学科建设工程资助项目(164320H106) (164320H106)

A novel hydrogen peroxide biosensor based on three-dimensionally ordered macroporous graphene-doped titanium dioxide modified ITO electrode(3DOM GR-Au-TiO₂/ITO) was prepared by a sol-gel procedure using polystyrene colloidal crystals as template.Catalase has been successfully immobilized on the modified electrode.The cyclic voltammetry(CV),electrochemical impedance spectra(EIS) and chronoamperometric characterizations reveal that the modified electrode exhibits an excellent electrocatalytic activity,a wide linear range for H₂O₂ concentration from 3.0×10^-6 mol/L to 3.6×10^-3 mol/L with a detection limit of 4.2×10^-7 mol/L(S/N=3),fast response,high sensitivity,good reproducibility and stability.
- catalase,
- graphene,
- nanometer titanium dioxide,
- biosensor,
- electrocatalysis
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