Citation: Wang Zhipeng, Li Man, Li Hui, Liu Zhihua, Li Ying, Zheng Ji-Shen. Chemical (Semi-) Synthesis and Applications of Lysine Post-Translationally Modified Proteins[J]. Chinese Journal of Organic Chemistry, ;2018, 38(9): 2400-2411. doi: 10.6023/cjoc201804046 shu

Chemical (Semi-) Synthesis and Applications of Lysine Post-Translationally Modified Proteins

Wang Zhipeng ^a,b,, ,
Li Man ^c ,
Li Hui ^d ,
Liu Zhihua ^c ,
Li Ying ^a ,
Zheng Ji-Shen ^a,,

a.
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
b.
Department of Chemistry, Texas A & M University, Texas 77840, USA
c.
Department of Plant Pathology and Microbiology, Texas A & M University, Texas 77843, USA
d.
Anhui Supervision of Veterinay Drug and Feed, Hefei 230091, China

Corresponding author: Wang Zhipeng, hipeng.wang@chem.tamu.edu Zheng Ji-Shen, jszheng@ustc.edu.cn
Received Date: 28 April 2018
Revised Date: 19 May 2018
Available Online: 7 September 2018
Fund Project: Project supported by the National Natural Science Foundation of China (No. U1732161), the Science and Technological Fund of Anhui Province for Outstanding Youth (No. 1808085J04) and the Innovative Program Development Foundation of Hefei Center Physical Science and Technology (No. 2017FXCX002)the National Natural Science Foundation of China U1732161the Innovative Program Development Foundation of Hefei Center Physical Science and Technology 2017FXCX002the Science and Technological Fund of Anhui Province for Outstanding Youth 1808085J04

Figures(9)

The study of native proteins with post-translational modifications (PTMs) is one of the main fields of epigenetics. The discovery of novel PTM models and their vital regulatory role for chromatin structure and gene transcription have been one of the current research focuses drawing attention of biologists especially in recent years. However, we still lack efficient strategies for the preparation of sufficient amount of native proteins with certain PTMs. The currently existing chemical biology methods are reviewed, and their advantages and disadvantages are compared, including bioorthogonal reaction technique, non-canonical amino acid incorporation, etc. Furthermore, the draft will mainly focus on the application of bioorthogonal reactions on unnatural functional groups for the incorporation of lysine PTMs.
- chemical synthesis of proteins,
- post-translational modifications,
- non-canonical amino acid,
- bioorthogonal reaction,
- proteome
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