Citation: Jin Tongyu, An Yu, Zhang Fan, He Pingang. Real-time Monitoring Skin Cell Alignment on Nano-grooves Using Electric Cell-substrate Impedance Sensing (ECIS)[J]. Acta Chimica Sinica, ;2017, 75(11): 1115-1120. doi: 10.6023/A17070337 shu

Real-time Monitoring Skin Cell Alignment on Nano-grooves Using Electric Cell-substrate Impedance Sensing (ECIS)

College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241

Corresponding author: Zhang Fan, fzhang@chem.ecnu.edu.cn He Pingang, pghe@chem.ecnu.edu.cn
Received Date: 26 July 2017
Available Online: 8 November 2017
Fund Project: the National Natural Science Foundation of China 21405049Project supported by the National Natural Science Foundation of China (No. 21405049)

Figures(9)

Cell alignment plays a crucial role in the repair and regeneration of tissues, which is caused by the "contact guidance" of micro/nano structures. In this paper, the nano-grooves with 200 nm in width, 400 nm in period and 75 nm in depth were fabricated on gold substrate with the technique of nanoimprint to simulate the extracellular matrix (ECM). Electric cell-substrate impedance sensing (ECIS) was employed firstly to real-time monitor cell alignment of human foreskin fibroblasts (HFF) and human immortal keratinocyte cells (HaCaT) on nano-grooves, which are two important functional cell types in skin wound-healing. The cell images displayed that the nano-grooves could induce the alignment of HFF cells, in which, the cell arrangement along the direction of nano-grooves occurred prior to the cell elongation. While the nano-grooves couldn't influence the morphology of HaCaT cells, and their adhesion and spread were delayed. In the ECIS monitoring, HFF and HaCaT cells both presented increased normalized impedance (NI) values at their respective characteristic frequencies of 977 and 1465 Hz on nano-grooves and flat electrodes with the prolongation of culture time during 24 h and the increasing trends of NI values were also similar:in the first 6 h, NI values increased faster, and then, the increasing rates declined obviously. HFF cells on nano-grooves generated more intense impedance signals with a larger distinction of increasing rate than those on flat electrodes, indicating that the nano-grooves could promote the adhesion and spread of HFF cells and the directional arrangement had a larger impact on the variation of NI values than cell elongation. While HFF cells adhered and spread in random directions, leading to the reduced difference in increasing rate of NI values. The NI values of HFF cells was further correlated with cell alignment, showing the enhanced impedance responses with the rising percentage of aligned cells. More importantly, there was a good linear correlation between NI values and the percentage of cells arranging along the direction of nano-grooves. In contrast, HaCaT cells had smaller NI values on the nano-grooves with the similar increasing rates, compared to the flat electrodes, revealing that the nano-grooves were less suitable for the adhesion and spread of HaCaT cells with almost no change of cell morphology, and the cell adhesion could cause more obvious variation of NI values than cell spread. Our research would provide a support for the development of complex cell sensors based on ECIS and its application in clinical research field.
- nano-grooves,
- cell alignment,
- electric cell-substrate impedance sensing (ECIS),
- skin cells,
- complex cell sensor
1. [1]
  Goodman, S. L.; Sims, P. A.; Albrecht, R. M. Biomaterials 1996, 17, 2087. doi: 10.1016/0142-9612(96)00016-6
2. [2]
  Gerecht, S.; Bettinger, C. J.; Zhang, Z. T.; Borenstein, J. T.; Vunjak-Novakovic, G.; Langer, R. Biomaterials 2007, 28, 4068. doi: 10.1016/j.biomaterials.2007.05.027
3. [3]
  Patel, S.; Kurpinski, K.; Quigley, R.; Gao, H. F.; Hsiao, B. S.; Poo, M. M.; Li, S. Nano Lett. 2007, 7, 2122 doi: 10.1021/nl071182z
4. [4]
  Flemming, R. G.; Murphy, C. J.; Abrams, G. A.; Goodman, S. L.; Nealey, P. F. Biomaterials 1999, 20, 573. doi: 10.1016/S0142-9612(98)00209-9
5. [5]
  Isenberg, B. C.; Tsuda, Y.; Williams, C.; Shimizu, T.; Yamato, M.; Okano, T.; Wong, J. Y. Biomaterials 2008, 29, 2565. doi: 10.1016/j.biomaterials.2008.02.023
6. [6]
  Kim, H. N.; Hong, Y.; Kim, M. S.; Kim, S. M.; Suh, K. Y. Bio-materials 2012, 33, 8782.
7. [7]
  Zhou, F.; Yuan, L.; Huang, H.; Chen, H. Chin. Sci. Bull. 2009, 54, 3200. doi: 10.1007/s11434-009-0366-1
8. [8]
  Choi, C. H.; Hagvall, S. H.; Wu, B. M.; Dunn, J. C. Y.; Beygui, R. E.; Kim, C. J. Biomaterials 2007, 28, 1672. doi: 10.1016/j.biomaterials.2006.11.031
9. [9]
  Crouch, A. S.; Miller, D.; Luebke, K. J.; Hu, W. Biomaterials 2009, 30, 1560. doi: 10.1016/j.biomaterials.2008.11.041
10. [10]
  Lamers, E.; Walboomers, X. F.; Domanski, I. M.; Riet, J.; Delft, F. C. M. J. M.; Luttge, R.; Winnubst, L. A. J. A.; Gardeniers, H. J. G. E.; Jansen, J. A. Biomaterials 2010, 31, 3307. doi: 10.1016/j.biomaterials.2010.01.034
11. [11]
  Tsai, W. B.; Ting, Y. C.; Yang, J. Y.; Lai, J. Y.; Liu, H. L. J. Mater. Sci-Mater. Med. 2009, 20, 1367. doi: 10.1007/s10856-008-3687-8
12. [12]
  Viswanathan, P.; Ondeck, M. G.; Chirasatitsin, S.; Ngamkham, K. Reilly, G. C.; Engler, A. J.; Battaglia, G. Biomaterials 2015, 52, 140. doi: 10.1016/j.biomaterials.2015.01.034
13. [13]
  Liu, G. F.; Zhang, D.; Feng, C. L. Angew. Chem., Int. Ed. 2014, 53, 7789. doi: 10.1002/anie.201403249
14. [14]
  Liu, X. L.; Wang, S. T. Chem. Soc. Rev. 2014, 43, 2385. doi: 10.1039/C3CS60419E
15. [15]
  Zong, X. H.; Bien, H.; Chung, C. Y.; Yin, L. H.; Fang, D. F.; Hsiao, B. S.; Chu, B.; Entcheva, E. Biomaterials 2005, 26, 5330. doi: 10.1016/j.biomaterials.2005.01.052
16. [16]
  Zhu, B. S.; Zhang, Q. Q.; Lu, Q. H.; Xu, Y. H.; Yin, J.; Hu, J.; Wang, Z. G. Biomaterials 2003, 25, 4215.
17. [17]
  Giaever, I.; Keese, C. R. Proc. Nat. Acad. Sci. U. S. A. 1984, 81, 3761. doi: 10.1073/pnas.81.12.3761
18. [18]
  Xiao, C.; Lachance, B.; Sunahara, G.; Luong, J. H. T. Anal. Chem. 2002, 74, 1333. doi: 10.1021/ac011104a
19. [19]
  Zhang, X.; Wang, W.; Li, F.; Voiculescu, I. Lab Chip 2017, 17, 2054. doi: 10.1039/C7LC00375G
20. [20]
  Abiri, H.; Abdolahad, M.; Gharooni, M.; Hosseini, S. A.; Janmaleki, M.; Azimi, S.; Hosseini, M.; Mohajerzadeh, S. Biosens. Bioelectron. 2015, 68, 577. doi: 10.1016/j.bios.2015.01.057
21. [21]
  Curtis, T. M.; Widder, M. W.; Brennan, L. M.; Schwager, S. J.; Schalie, W. H.; Fey, J.; Salazar, N. Lab Chip. 2009, 9, 2176. doi: 10.1039/b901314h
22. [22]
  Asphahani, F.; Zhang, M. G. Analyst 2007, 132, 835. doi: 10.1039/b704513a
23. [23]
  Xiao, C. D.; Lachance, B.; Sunahara, G.; Luong, J. H. T. Anal. Chem. 2002, 74, 5748. doi: 10.1021/ac025848f
24. [24]
  Xie, F. B.; Xu, Y. C.; Wang, L.; Mitchelson, K.; Xing, W. L.; Cheng, J. Analyst 2012, 137, 1343. doi: 10.1039/c2an16141a
25. [25]
  Kang, G.; Kim, Y. J.; Moon, H. S.; Lee, J. W.; Yoo, T. K.; Park, K.; Lee, J. H. Biomicrofluidics 2013, 7, 044126. doi: 10.1063/1.4818838
26. [26]
  Yang, L. J.; Arias, L. R.; Lane, T. S.; Yancey, M. D.; Mamouni, J. Anal. Bioanal. Chem. 2011, 399, 1823. doi: 10.1007/s00216-010-4584-9
27. [27]
  Yim, E. K. F.; Pang, S. W.; Leong, K. W. Exp. Cell Res. 2007, 313, 1820. doi: 10.1016/j.yexcr.2007.02.031
28. [28]
  Solly, K.; Wang, X. B.; Xu, X.; Strulovici, B.; Zheng, W. Assay Drug Dev. Techn. 2004, 2, 363. doi: 10.1089/adt.2004.2.363
29. [29]
  Cui, Y.; An, Y.; Jin, T. Y.; Zhang, F.; He, P. G. Sensor. Actuat. B-Chem. 2017, 250, 461. doi: 10.1016/j.snb.2017.04.183
30. [30]
  Hug, T. S. Assay Drug Dev. Techn. 2003, 1, 479. doi: 10.1089/154065803322163795
31. [31]
  Zhao, H. Ph.D. Dissertation, East China Normal University, Shanghai, 2012(in Chinese).
1. [1]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
2. [2]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 2310046-0. doi: 10.3866/PKU.WHXB202310046
3. [3]
  Zhaoxin LI , Ruibo WEI , Min ZHANG , Zefeng WANG , Jing ZHENG , Jianbo LIU . Advancements in the construction of inorganic protocells and their cell mimic and bio-catalytical applications. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2286-2302. doi: 10.11862/CJIC.20240235
4. [4]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
5. [5]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . Photomultiplication-Type All-Polymer Photodetectors and Their Applications in Photoplethysmography Sensor. Acta Physico-Chimica Sinica, 2025, 41(1): 100007-0. doi: 10.3866/PKU.WHXB202311021
6. [6]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
7. [7]
  Xiaojing Tian , Zhichun Huang , Qingsong Zhang , Xu Wang , Ning Yang , Nanping Deng . PNIPAm Thermo-Responsive Nanofibers Mats: Morphological Stability and Response Behavior under Cross-Linking. Acta Physico-Chimica Sinica, 2024, 40(4): 2304037-0. doi: 10.3866/PKU.WHXB202304037
8. [8]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
9. [9]
  Shipeng WANG , Shangyu XIE , Luxian LIANG , Xuehong WANG , Jie WEI , Deqiang WANG . Piezoelectric effect of Mn, Bi co-doped sodium niobate for promoting cell proliferation and bacteriostasis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1919-1931. doi: 10.11862/CJIC.20240094
10. [10]
  Peng GENG , Guangcan XIANG , Wen ZHANG , Haichuang LAN , Shuzhang XIAO . Hollow copper sulfide loaded protoporphyrin for photothermal-sonodynamic therapy of cancer cells. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1903-1910. doi: 10.11862/CJIC.20240155
11. [11]
  Jiahao Zeng , Hui Chao . 诱导程序性细胞死亡的金属抗肿瘤药物研究. University Chemistry, 2025, 40(6): 145-159. doi: 10.12461/PKU.DXHX202406019
12. [12]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
13. [13]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
14. [14]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
15. [15]
  Yanxi LIU , Mengjia XU , Haonan CHEN , Quan LIU , Yuming ZHANG . A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1112-1122. doi: 10.11862/CJIC.20240423
16. [16]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
17. [17]
  Xiao-Qi Xu , Yapei Wang . Practice of Cultivating Multi-Disciplinary Talents with Comprehensive Skills through Demand-Driven, Individualized Education, and Humanities and Science Integration. University Chemistry, 2024, 39(6): 90-97. doi: 10.3866/PKU.DXHX202311049
18. [18]
  Hui Xiong , Yan Wang , Rongxian Bai , Yongqi Wu , Chengmei Liu , Yuefa Gong , Jian Zhang . Development of a Compound Talent Training System Based on Virtual Technology: a Case Study of Chemical Unit and Process Simulation Practices. University Chemistry, 2024, 39(10): 314-317. doi: 10.12461/PKU.DXHX202405071
19. [19]
  Yumiao Gao , Yixin Chen , Jiaxin Wei , Junjie Yu , Yunxia Wang . Guarding the Kingdom: Skin Allies with Sunscreen for Mutual Protection. University Chemistry, 2024, 39(9): 74-80. doi: 10.12461/PKU.DXHX202404149
20. [20]
  Yuping Wei , Yiting Wang , Jialiang Jiang , Jinxuan Deng , Hong Zhang , Xiaofei Ma , Junjie Li . Interdisciplinary Teaching Practice——Flexible Wearable Electronic Skin for Low-Temperature Environments. University Chemistry, 2024, 39(10): 261-270. doi: 10.12461/PKU.DXHX202404007

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(2185)
HTML views(233)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142