Citation: LIU Yuan, LONG Mei, XIE Meng-Xia. Mechanism of Interaction between Chrysin and Different Configurations of Human Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2013, 29(12): 2647-2654. doi: 10.3866/PKU.WHXB201310311 shu

Mechanism of Interaction between Chrysin and Different Configurations of Human Serum Albumin

1.
1 Analytical & Testing Center of Beijing Normal University, Beijing 100875, P. R. China;
2 Department of Chemistry and Chemical Technology, Chifeng University, Chifeng 024000, Inner Mon lia Autonomous Region, P. R. China

Received Date: 28 June 2013
Available Online: 31 October 2013
Fund Project: 内蒙古自治区自然科学基金(2012MS0212)资助项目 (2012MS0212)

The mechanism of the interaction between Chrysin (CHR) and human serum albumin (HSA) was investigated using various spectroscopic techniques. It was found that CHR can induce HSA fluorescence emission quenching by static process. Additionally, HSA caused a significant red shift in the ultraviolet absorption of CHR. This indicated that binding with a protein can result in dissociation of the phenolic hydroxyl in CHR. HSA can also cause CHR fluorescence emission. The binding constants of CHR and HAS were calculated based on the fluorescence quenching and emission modes. The results obtained using these two methods were consistent. The binding constant (K_A) values at pH 7.4 were (9.97±0.24)×10⁴ and (9.75±0.11)×10⁴ L·mol^-1, respectively; and the ratio of combination was 1:1. The binding constants decreased gradually with decreasing the pH value. This is related to conformational changes in the protein. The protein is partly denatured at pH 3.5, with the site Ⅱ in the HSA opening chain. It was found that CHR was located at site I, which is in subdomain ⅡA of HAS. Based on molecular modeling, the mechanism of the interaction between CHR and HAS was investigated.
- Chrysin,
- Human serum albumin,
- Fluorescence,
- pH effect,
- Molecular modeling
1. [1]
  
  (1) Soares, S.; Mateus, N.; Freitas, V. D. J. Agric. Food Chem.2007, 55, 6726. doi: 10.1021/jf070905x
2. [2]
  (2) Yang, Y. M.; Hu, Q. L.; Fan, Y. L.; Shen, H. S. SpectrochimicaActa Part A 2008, 69, 432. doi: 10.1016/j.saa.2007.04.019
3. [3]
  (3) Li, D. J.; Zhu, J. F.; Jin, J.; Yao, X. J. Journal of MolecularStructure 2007, 846, 34. doi: 10.1016/j.molstruc.2007.01.020
4. [4]
  (4) Kanakias, C. D.; Nafisi, S.; Rajai, M.; Shadaloi, A.; Tarantilis, P.A.; Polissiou, M. J.; Bariyanga, J.; Tajmir-Riahi, H. A.Spectroscopy 2009, 23, 29. doi: 10.1155/2009/154321
5. [5]
  (5) Ma, J.; Liu, Y.; Xie, M. X. Spectroscopy and Spectral Analysis2012, 32 (1), 1. [马纪, 刘媛, 谢孟峡. 光谱学与光谱分析, 2012, 32 (1), 1.]
6. [6]
  (6) Ma, G. Z.; Tan, F.; Jiang, Y. J.; Zheng, K.W.; Guo, M.; Yu, Q.C. Acta Phys. -Chim. Sin. 2005, 21 (2), 123. [马国正, 谭非,蒋勇军, 郑柯文, 郭明, 俞庆森. 物理化学学报, 2005, 21 (2),123.] doi: 10.3866/PKU.WHXB20050203
7. [7]
  (7) Guo, Q. L.; Li, R.; Jiang, F. L.; Tu, J. C.; Li, L.W.; Liu, Y. ActaPhys. -Chim. Sin. 2009, 25 (10), 2147. [郭清莲, 李冉, 蒋风雷, 涂建成, 李林尉, 刘义. 物理化学学报, 2009, 25 (10),2147.] doi: 10.3866/PKU.WHXB20091112
8. [8]
  (8) Twine, S. M.; re, M. G.; Morton, P. Arch. Biochem. Biophys.2003, 414, 83. doi: 10.1016/S0003-9861(03)00173-5
9. [9]
  (9) Yamasaki, K.; Maruyama, T.; Yoshimoto, K.; Tsutsumi, Y.Biochim. Biophys. Acta 1999, 1432, 313. doi: 10.1016/S0167-4838(99)00098-9
10. [10]
  (10) Ahmad, B.; Ankita; Khan, R. H. Arch. Biochem. Biophys. 2005,437, 159. doi: 10.1016/j.abb.2005.03.013
11. [11]
  (11) Sakai, T.; Yamasaki, K.; Sako, T.; Kragh, H. U.; Suenaga, A.;Otagiri, M. Pharm. Res. 2001, 18 (4), 520. doi: 10.1023/A:1011014629551
12. [12]
  (12) Zanoli, P.; Avalloneb, R.; Baraldi, M. Fitoterapia 2000, 71,117. doi: 10.1016/S0367-326X(00)00186-6
13. [13]
  (13) Castro, G. T.; Ferretti, F. H.; Blanco, S. E. Spectrochimica ActaPart A 2005, 62, 657. doi: 10.1016/j.saa.2005.02.013
14. [14]
  (14) Friedman, M.; Jurgens, H. S. J. Agric. Food Chem. 2000, 48,2101. doi: 10.1021/jf990489j
15. [15]
  (15) Tommasini, S.; Calabro, M. L.; Donato, P. J. Pharm. Biomed.Anal. 2004, 35, 389. doi: 10.1016/S0731-7085(03)00586-7
16. [16]
  (16) Qin, C.; Xie, M. X.; Liu, Y. Biomacromolecules 2007, 8,2182. doi: 10.1021/bm070319c
17. [17]
  (17) Liu, Y.; Xie, M. X.; Jiang, M.;Wang, Y. D. SpectrochimicaActa Part A 2005, 61, 2245. doi: 10.1016/j.saa.2004.09.004
18. [18]
  (18) Rasoulzadeh, F.; Jabary, H. N.; Naseri, A.; Rashidi, M. R.Spectrochimica Acta Part A 2009, 72, 190. doi: 10.1016/j.saa.2008.09.021
19. [19]
  (19) Yu, Z. L.; Li, D. J.; Ji, B. M.; Chen, J. J. Journal of MolecularStructure 2008, 889, 422. doi: 10.1016/j.molstruc.2008.03.013
20. [20]
  (20) Papadopoulou, A.; Green, R. J.; Frazier, R. A. J. Agric. FoodChem. 2005, 53, 158. doi: 10.1021/jf048693g
21. [21]
  (21) Maurice, R.; Eftink; Camillo, A.; Ghiron. Analyt. Biochem.1981, 114, 199. doi: 10.1016/0003-2697(81)90474-7
22. [22]
  (22) Lakowicz, J. R. Principles of Fluorescence Spectroscopy;Plenum Press: New York, 1983; p 257.
23. [23]
  (23) Fang, R.; Leng, X. J.;Wu, X.; Li, Q.; Hao, R. F.; Ren, F. Z.;Jing, H. Spectroscopy and Spectral Analysis 2012, 32 (1), 108.[方茹, 冷小京, 吴夏, 李琦, 郝瑞芳, 任发政, 景浩.光谱学与光谱分析, 2012, 32 (1), 108.]
24. [24]
  (24) Bhattacharya, J.; Bhattacharya, M.; Chakrabarty, A. S.;Chaudhury, U.; Poddar, R. K. Biochem. Pharmacology 1994,47, 2049. doi: 10.1016/0006-2952(94)90080-9
25. [25]
  (25) Sytnik, A.; Lritvinyuk, I. Proceedings of the National Academyof Sciences of the United States of America 1996, 93 (23),12959. doi: 10.1073/pnas.93.23.12959
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