Citation: WANG Yan-Yan, JIANG Yan-Xia, ROGACH Andrey, SUN Shi-Gang. Effect of pH and Au Nanoparticles on Cytochrome c Investigated by Electrochemistry and UV-Vis Absorption Spectroscopy[J]. Acta Physico-Chimica Sinica, ;2012, 28(05): 1127-1133. doi: 10.3866/PKU.WHXB201203073 shu

Effect of pH and Au Nanoparticles on Cytochrome c Investigated by Electrochemistry and UV-Vis Absorption Spectroscopy

1.
1. Key Laboratory of Physical Chemistry of Solid Surface, Department of Chemistry, College of Chemistry and Chemistry Engineering, Xiamen University, Xiamen 361005, Fujian Province, P. R. China;
2. Department of Physics and Materials Science, City University of Hong Kong, Hong Kong, P. R. China

Received Date: 13 January 2012
Available Online: 7 March 2012
Fund Project: 国家自然科学基金(20833005, 20873116, 60936003, 21021002)资助项目 (20833005, 20873116, 60936003, 21021002)

In this paper, ld nanoparticles (NPs) with an average size of (4.7 ± 0.6) nm, capped with mercaptopropionic acid (MPA) ligand, are prepared. The effect of pH and Au NPs on cytochrome c (Cyt c) is investigated by electrochemistry and UV-Vis absorption spectroscopy. UV-Vis absorption spectra indicate that the structures of Cyt c and Cyt c-Au NPs complex do not change appreciably between pH=7.5 and pH=3.0, but their Soret band positions change markedly at pH=2.0, indicating that low pH value induces a conformational change in Cyt c. Cyclic voltammetry (CV) result shows that Au NPs capped with MPA enhance electron transfer between Cyt c and the electrode. The data also reveals that the biocompatibility of Au NPs is improved when citric acid ligand is replaced with MPA. The change in pH value causes a change of peak currents in CV and a shift of peak potential. When pH value deviates from 7.0, levels of electroactive Cyt c decrease. Significant pH change induces irreversible denaturing of Cyt c. The pH at which Cyt c-Au NPs complex denatures completely is one unit higher than that of Cyt c. Combining the results from UV-Vis spectroscopy and CV, we find that addition of Au NPs makes adsorbing state Cyt c more vulnerable to pH.
- Au nanoparticle,
- Cytochrome c,
- pH value,
- Cyclic voltammetry,
- UV-Vis absorption spectroscopy
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