Citation: PENG Yu-Ling, WANG Shu-Jun, FU Li, ZHANG Cheng-Gen, LIU Xin-Gang. Synthesis of Tailed Porphyrin Modified with Nicotinic Acid and Interactions with Human Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2012, 28(05): 1054-1062. doi: 10.3866/PKU.WHXB201202222 shu

Synthesis of Tailed Porphyrin Modified with Nicotinic Acid and Interactions with Human Serum Albumin

1.
College of Chemistry & Material Science, Langfang Teachers College, Langfang 065000, Hebei Province, P. R. China

Received Date: 29 November 2011
Available Online: 22 February 2012
Fund Project: 河北省自然科学基金(B2010001803) (B2010001803) 廊坊师范学院科学研究项目重点专项基金(LSZZ201003)资助 (LSZZ201003)

Free porphyrins, namely o-(niacin)C₂O-T(3p-OCH₃)PP and p-(niacin)C₂O-T(3p-OCH₃)PP, their complexes, o-(niacin)C₂O-T(3p-OCH₃)PPZn and p-(niacin)C₂O-T(3p-OCH₃)PPZn modified with nicotinic acid, were designed, synthesized, and characterized by elementary analysis, and UV-Vis, 1H nuclear magnetic responance (¹H NMR), and infrared (IR) spectroscopies. The conformations of the Zn porphyrins were calculated using a quantum- chemical method. The experimental results showed the following. It was found that the nicotinic acid group was on the porphyrin plane in o-(niacin)C₂O-T(3p-OCH₃)PPZn and Zn― N intramolecular coordination interactions which existed between the N atom of the nicotinic acid group in the side-chain and the Zn²⁺ in the porphyrin plane. The nicotinic acid group was far from the porphyrin plane in p-(niacin)C₂O-T(3p-OCH₃)PPZn and Zn ― N intermolecular coordination interactions which existed between the N atom of the nicotinic acid group in one Zn porphyrin and the Zn²⁺ in the other porphyrin plane. The fluorescence properties of the interactions between Zn porphyrins and human serum albumin (HSA) were studied using fluorescence spectroscopy. There is a large quenching interaction between the Zn porphyrins and human serum albumin. The mechanism of the combination reaction is hydrogen bonding or van der Waals interactions between the Zn porphyrins and human serum albumin. The fluorescence quenching data were analyzed using the Stem-Volmer equation and a double-reciprocal equation, and the quenching constant, binding constant, and thermodynamic parameters were obtained．
- Nicotinic acid,
- Zn porphyrin,
- Human serum albumin,
- Fluorescence quenching,
- Thermodynamic parameter
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