Citation: DENG Lin, QI Zhi-Mei. Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy[J]. Acta Physico-Chimica Sinica, ;2010, 26(10): 2672-2678. doi: 10.3866/PKU.WHXB20101003 shu

Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy

DENG Lin ,
QI Zhi-Mei

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received Date: 24 May 2010
Available Online: 27 September 2010
Fund Project: 国家重点基础研究发展计划(973)(2009CB320300) (973)(2009CB320300)国家自然科学基金(60978042) (60978042)

We used time-resolved optical waveguide spectroscopy (OWGS) to in-situ measure visible absorption spectrum for methylene blue (MB) adsorbed ona hydrophilic glass waveguide from an aqueous solution containing proteins such as bovine serum albumin (BSA) and hemoglobin (Hb). The competitive adsorption of MB and the protein was detected at a solution pH lower than the isoelectric point of the protein. Since the competitive adsorption behavior of MB and the protein is very sensitive to the protein concentration, the protein content in the mixed solution was readily determined by time-resolved OWGS. In addition, a kinetic equation for the competitive adsorption of the two molecules was deduced based on the Langmuir adsorption isotherm. The best fit of the measured time course of the absorbance with the theoretical kinetic equation reveals that the number of MB molecules adsorbed on the surface exponentially decreases with time after reaching a maximum.A quasi-linear relationship between the fitting parameters and the protein concentration was also obtained.
- Optical waveguide spectroscopy,
- Competitive adsorption,
- Methylene blue,
- Protein detection
1. [1]
  
  1. Marco, A.; Rubio, R.; Compano, R.; Casals, I. Talanta, 2002, 57: 1019
2. [2]
  2. Coakley, W. T.; James, C. J. Anal. Biochem., 1978, 85: 90
3. [3]
  3. Shen, W.; Chen, J. Z.; Dai, C. J. Food Res. Dev., 2009, 30: 96 [沈文,陈均志,代春吉. 食品研究与开发, 2009, 30: 96]
4. [4]
  4. Li, J.; Zhang, Y. T.; Zeng, W.; Luo, X.; Liao, C. C. Chin. J. Biol., 2000, 13: 118 [李娟,张耀庭,曾伟,罗璇,廖长春.中国生物制品学杂志, 2000, 13: 118]
5. [5]
  5. Minagawa, M.; Winter, D. A.; Kaplan, I. R. Anal. Chem., 1984, 56: 1859
6. [6]
  6. Penninckx,W.; Verbeke, J. S.; Vankeerberghen, P.; Massart, D. L. Anal. Chem., 1996, 68: 481
7. [7]
  7. Yu, X. L.; Wei, X.; Wang, D. X.; Ding, X.; Liao, W.; Zhao, X. S. Acta Phys.-Chim. Sin., 2005, 21: 888 [余兴龙,魏星,王鼎新, 定翔,廖伟,赵新生.物理化学学报, 2005, 21: 888]
8. [8]
  8. Schmitt, K.; Schirmer, B.; Hoffmann, C.; Brandenburg, A.; Meyrueis, P. Biosens. Bioelectro., 2007, 22: 2591
9. [9]
  9. Wu, Z.W.; Liu, Q. J.; Wu, L. W.; Xie, X.; Zhuang, Y.;Wen, T.; Bai, Y. F.; Lu, Z. H. Acta Optica Sinica, 2007, 27: 521 [吴中伟, 刘全俊,吴凌伟, 谢骁,庄萤,温恬, 白云飞, 陆祖宏.光学学报, 2007, 27: 521]
10. [10]
  10. Kim, N.; Kim, D. K.; Kim, W. Y. Food Chem., 2008, 108: 768
11. [11]
  11. Enami, Y.; Fukuda, T.; Suye, S. Appl. Phys. Lett., 2007, 91: 203507-1
12. [12]
  12. Kim, N.; Kim, D. Y.; Cho, Y. J.; Moon, D. K.; Kim, W. Y. Biosens. Bioelectro., 2008, 24: 391
13. [13]
  13. Choquette, S. J.; Browm, L. L.; Durst, R. A. Anal. Chem., 1992, 64: 55
14. [14]
  14. Bradshaw, J. T.; Mendes, S. B.; Saavedra, S. S. Anal. Chem., 2005: 29A
15. [15]
  15. Qi, Z. M.; Matsuda, N.; Takatsu, A.; Kato, K. J. Phys. Chem. B, 2003, 107: 6873
16. [16]
  16. Cameron, P. J.; Jenkins, A. T. A.; Knoll, W.; Marken, F.; Milsom, E. V.; Williams, T. L. J. Mater. Chem., 2008, 18: 4304
17. [17]
  17. Qi, Z. M.; Xia, S. H.; Matsuda, N. Anal. Biochem., 2008, 374: 196
18. [18]
  18. Mendes, S. B.; Saavedra, S. S. Opt. Express, 1999, 4: 449
19. [19]
  19. Fujita, K.; Taniguchi, K.; Ohno, H. Talanta, 2005, 65: 1066
20. [20]
  20. Deng, L.; Lu, D. F.; Qi, Z. M. Acta Phys.-Chim. Sin., 2009, 25: 2481 [邓琳,逯丹凤,祁志美. 物理化学学报, 2009, 25: 2481]
21. [21]
  21. Azizian, S.; Haerifar, M.; Parsa, J. B. Chemosphere, 2007, 68: 2040
22. [22]
  22. mri, S.; Seguin, J. L.; Guerin, J.; Aguir, K. Sens. Actuators, 2006, 114: 451
23. [23]
  23. Koutsopoulos, S.; Patzsch, K.; Bosker,W. T. E.; Norde, W. Langmuir, 2007, 23: 2000
24. [24]
  24. Kusakari, A.; Izumi, M.; Ohnuki, H. Colloids Surf. A, 2008, 321: 47
25. [25]
  25. Can, O.; Holland, N. B. J. Colloid Interface Sci., 2009, 329: 24
1. [1]
  Xinran Zhang , Siqi Liu , Yichi Chen , Qingli Zou , Qinghong Xu , Yaqin Huang . From Protein to Energy Storage Materials: Edible Gelatin Jelly Electrolyte. University Chemistry, 2025, 40(7): 255-266. doi: 10.12461/PKU.DXHX202408104
2. [2]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
3. [3]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
4. [4]
  Yichang Liu , Li An , Dan Qu , Zaicheng Sun . “双碳”背景下的综合设计实验——以PbCrO₄催化甲基蓝的光降解速率常数测定为例. University Chemistry, 2025, 40(6): 222-229. doi: 10.12461/PKU.DXHX202407105
5. [5]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
6. [6]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
7. [7]
  Yan Yuan , Haitao Wu , Yi Zhang , Li Jiang , Feng Cao , Yanmao Dong . Research on the Talent Training System to Enhance the Core Competence of Employment for Undergraduate Students Majoring in Materials Chemistry. University Chemistry, 2024, 39(11): 52-56. doi: 10.12461/PKU.DXHX202402015
8. [8]
  Jinghui Zhang , Wei Shen , Sheng Tang , Ru Jia , Wei Zhong . Exploration and Reflection on Interdisciplinary Teaching of Analytical Chemistry in the New Era of International Competition. University Chemistry, 2025, 40(7): 1-9. doi: 10.12461/PKU.DXHX202408019
9. [9]
  Jianan Hong , Chenyu Xu , Yan Liu , Changqi Li , Menglin Wang , Yanwei Zhang . Decoding the interfacial competition between hydrogen evolution and CO₂ reduction via edge-active-site modulation in photothermal catalysis. Acta Physico-Chimica Sinica, 2025, 41(9): 100099-0. doi: 10.1016/j.actphy.2025.100099
10. [10]
  Chi Li , Jichao Wan , Qiyu Long , Hui Lv , Ying Xiong . N-Heterocyclic Carbene (NHC)-Catalyzed Amidation of Aldehydes with Nitroso Compounds. University Chemistry, 2024, 39(5): 388-395. doi: 10.3866/PKU.DXHX202312016
11. [11]
  Xiaosong PU , Hangkai WU , Taohong LI , Huijuan LI , Shouqing LIU , Yuanbo HUANG , Xuemei LI . Adsorption performance and removal mechanism of Cd(Ⅱ) in water by magnesium modified carbon foam. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1537-1548. doi: 10.11862/CJIC.20240030
12. [12]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
13. [13]
  Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
14. [14]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
15. [15]
  Jiali CHEN , Guoxiang ZHAO , Yayu YAN , Wanting XIA , Qiaohong LI , Jian ZHANG . Machine learning exploring the adsorption of electronic gases on zeolite molecular sieves. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 155-164. doi: 10.11862/CJIC.20240408
16. [16]
  Yang ZHOU , Lili YAN , Wenjuan ZHANG , Pinhua RAO . Thermal regeneration of biogas residue biochar and the ammonia nitrogen adsorption properties. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1574-1588. doi: 10.11862/CJIC.20250032
17. [17]
  Xinhao Yan , Guoliang Hu , Ruixi Chen , Hongyu Liu , Qizhi Yao , Jiao Li , Lingling Li . Polyethylene Glycol-Ammonium Sulfate-Nitroso R Salt System for the Separation of Cobalt (II). University Chemistry, 2024, 39(6): 287-294. doi: 10.3866/PKU.DXHX202310073
18. [18]
  Jianan Zhang , Mengzhen Xu , Jiamin Liu , Yufei He . 面向“双碳”目标的脱氯吸附剂开发研究型综合实验设计. University Chemistry, 2025, 40(6): 248-255. doi: 10.12461/PKU.DXHX202408068
19. [19]
  Xueqi Yang , Juntao Zhao , Jiawei Ye , Desen Zhou , Tingmin Di , Jun Zhang . 调节NNU-55(Fe)的d带中心以增强CO₂吸附和光催化活性. Acta Physico-Chimica Sinica, 2025, 41(7): 100074-0. doi: 10.1016/j.actphy.2025.100074
20. [20]
  Fei Xie , Chengcheng Yuan , Haiyan Tan , Alireza Z. Moshfegh , Bicheng Zhu , Jiaguo Yu . d-Band Center Regulated O₂ Adsorption on Transition Metal Single Atoms Loaded COF: A DFT Study. Acta Physico-Chimica Sinica, 2024, 40(11): 2407013-0. doi: 10.3866/PKU.WHXB202407013

Get Citation

PDF

XML

Metrics

PDF Downloads(1271)
Abstract views(3045)
HTML views(41)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142