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Citation: YIN Yan-Dong, ZHOU Xiao-Xue, ZHAO Xin-Sheng. Maximum Entropy Method for Analyses of Fluorescence Correlation Spectra of Oli nucleotide Intra-Chain Collision[J]. Acta Physico-Chimica Sinica, ;2010, 26(04): 1087-1092. doi: 10.3866/PKU.WHXB20100408 shu

Maximum Entropy Method for Analyses of Fluorescence Correlation Spectra of Oli nucleotide Intra-Chain Collision

1.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China

Received Date: 26 October 2009
Available Online: 5 February 2010

It has been a challenge to accurately extract dynamic information from experimental fluorescence correlation spectroscopy (FCS) data. In this paper, we compare three major fitting methods: the model-dependent multiple exponential function (MultiExp), the empirical stretched exponential function (StreExp), and the exponential function based on the model-free maximumentropy method (MemExp). MultiExp has straight forward physical significance but it is difficult to implement and interpret in a complex system. StreExp has simple form and is easy to use but its physical picture is obscure. MultiExp is model free but its results are sensitive to experimental noise. A od choice seems to be a combination of MemExp and MultiExp. In our example, we have unraveled that two independent processes exist in the intra-chain collision of a single-stranded DNA when base pair formation is possible, which has not been observed by previous investigators. MemExp is recommended for the FCS data analysis, although caution should be exercised in the practice.
- Fluorescence correlation spectroscopy,
- Relaxation time distribution,
- Stretched exponential function,
- Maximumentropy method,
- Oli nucleotide,
- Photoinduced electron transfer
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