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Citation: GUO Xiao-Li, GUO Min, WANG Xin-Dong. Bienzymatic Glucose Biosensor Based on Graphite Electrode Modified with Nanofibrous Polyaniline[J]. Acta Physico-Chimica Sinica, ;2007, 23(04): 585-589. doi: 10.3866/PKU.WHXB20070426 shu

Bienzymatic Glucose Biosensor Based on Graphite Electrode Modified with Nanofibrous Polyaniline

1.
Department of Physical Chemistry, University of Science and Technology Beijing, Beijing 100083, P. R. China

Received Date: 26 September 2006
Available Online: 6 March 2007

Nanofibrous polyaniline (PANI) was electropolymeried on graphite electrode by cyclic voltammetry (CV), and a glucose bienzyme sensor based on glucose oxidase ( D) and horseradish peroxidase (HRP) immobilized on the modified electrode was fabricated. The bienzymatic sensor was charaterized by ac impedance spectrometry and SEM. The stability and the factors affected the response of the bienzymetic sensor to glucose were studied. The detection of glucose was carried out at 0.05 V (vs SCE) to minimize the electroactive interferences. The linear response range of the as-prepared bienzyme sensor for glucose was between 0.05 mmol·L-1 and 2.0 mmol·L-1.
- 纳米纤维聚苯胺; 双酶; 葡萄糖传感器
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