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Citation: Chen Zhi-Chun, Xu Shan-Hao, Lin Han-Feng, Yang Shao-Ming, Lin Xian-Fu. Glucose Oxidase Modified Electrode Fabricated by Layer-by-layer Molecular Deposition[J]. Acta Physico-Chimica Sinica, ;2004, 20(10): 1267-1270. doi: 10.3866/PKU.WHXB20041021 shu

Glucose Oxidase Modified Electrode Fabricated by Layer-by-layer Molecular Deposition

1.
Department of Chemistry ,Zhejiang University, Hangzhou　310027

Received Date: 20 April 2004
Available Online: 15 October 2004

In this paper,glucose oxidase( D) electrodes were fabricated by alternative adsorption of oppositely charged polycations (poly(dimethydiallyammonium)hydrochloride, PDDA) and enzymes on Au modified by 3-mercapto-1-propanesulfonic acid, sodium salt (MPS). The concentration of glucose was detected by cyclic voltammetry via ferrocene monocarboxylic acid as mediator. A linear relationship existed between peak current and concentration of glucose in the range 0.55～6.63 mmol•L－1. The effect of pH on the response current of electroroxidation of glucose by D had been studied and the optimum pH was 7.0.In addition, the biosensor showed od reproducibility for the determination of glucose and the relative standard deviation was 2.4％.
- Layer-by-layer assembly;Glucose oxidase ( D);Biosensor
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