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Citation: Li Yang-Mei, Jiang Xiu-Ming, Chen Zhi-Chun, Fu Shui-Yu, Lin Xian-Fu. Construction of Ordered Films of Horseradish Peroxidase through Lectin-glycogenReaction and Its Application in Enzyme Electrode[J]. Acta Physico-Chimica Sinica, ;2004, 20(02): 216-220. doi: 10.3866/PKU.WHXB20040223 shu

Construction of Ordered Films of Horseradish Peroxidase through Lectin-glycogenReaction and Its Application in Enzyme Electrode

1.
Chemistry Department of Zhejiang University, Hangzhou　310027; Chemical Engineering Department of Hangzhou Professional Technical College, Hangzhou　310018; Chemistry Department of Hangzhou Teachers College, Hangzhou　310012

Received Date: 5 August 2003
Available Online: 15 February 2004

The multilayer films of horseradish peroxidase (HRP) were fabricated by layer-by-layer assembly of HRP and concanavalin A (Con A) through lectin-glycogen reaction. The surface topography and roughness were characterized by AFM. The thickness of the films was studied by AFM and ellipsometry. The thicknesses of the Con A and the HRP film are about 9.0 and 4.6 nm, respectively, which correspond to their X-ray diffraction data. It is demonstrated that the shape of Con A and HRP molecule was well retained during the assembly process. Cyclic voltammetry (CV) was used to test the electrochemical properties of the films. The enzyme electrode of three bilayers of （Con A/HRP） films was sensitive for the reduction of hydrogen peroxide in the methylene blue (MB) solution. The response current is proportional to the concentration of H2O2 within the range of 0.2～1.0 mmol•L－1. The sensitivity of the enzyme electrode is 24.0 mA•mol－1 •L and the apparent Michaelis constant is 4.2 mmol•L－1.
- HRP;Con A;Surface topography;Assembly film;AFM;Enzyme electrode
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