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Citation: Li Yang-Mei, Chen Zhi-Chun, Lü De-Shui, Lin Xian-Fu. Topography and Electro-catalysis Property of Horseradish Peroxidase Assembly Active Films[J]. Acta Physico-Chimica Sinica, ;2003, 19(05): 474-477. doi: 10.3866/PKU.WHXB20030520 shu

Topography and Electro-catalysis Property of Horseradish Peroxidase Assembly Active Films

1.
Chemistry Department of Zhejiang University, Hangzhou　310027;Chemical Engineering Department of Hangzhou Professional Technical College, Hangzhou　310018

Received Date: 10 September 2002
Available Online: 15 May 2003

The multilayer films of horseradish peroxidase(HRP) were fabricated by layer-by-layer assembly of HRP and poly(sodium-p-styrenesulfonate)(PSS) on PET(polyethylene glycol terephthalate) and ld electrode surface. The activity of the assembly films was investigated. The surface topography was characterized by atomic force microscope (AFM). The relationship between the activity of the immobilized HRP with the surface roughness and topography was also studies. Cyclic voltammetry (CV) was used for the study of electrochemical properties of the films. The enzyme electrode of mltilayer films was sensitive for the reduction of hydrogen peroxide in the methylene blue (MB) solution. The response current is linear with the concentration of H2O2 in the concentration range of 0.2～5.0 mmol•L－1.
- Horseradish peroxidase(HRP);Self-assembled monolayer;Atomic force microscope(AFM);Enzyme electrode;H₂O₂
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