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Citation: Ding Ke-Qiang, He Xiao-Ling, Wang Qing-Fei, Jiang Dian-Lu, Bai Juan, Tong Ru-Ting, Wang Xin-Kui. The AC Impedance Study of Calmodulin Modified on Au Electrode[J]. Acta Physico-Chimica Sinica, ;2002, 18(08): 741-745. doi: 10.3866/PKU.WHXB20020814 shu

The AC Impedance Study of Calmodulin Modified on Au Electrode

1.
Chemistry College, Hebei Normal University, Shijiazhuang　050016;Institute of Coal Chemistry, Chinese Academy of Science, Taiyuan　030001

Received Date: 17 December 2001
Available Online: 15 August 2002

Calmodulin(CaM) as a major intracellular Ca2＋ receptor, was self-assembled on Au electrode and investigated by electrochemical impedance spectroscopy(EIS). The results demonstrated that the self-assembly process of CaM could be divided into rapid and slow steps clearly. The influence of pH value on the new modified electrode was found to be regular, and the most packed structure appeared at the calmodulin’s isoelectric-point, the combining process of calmodulin with Ca2＋ was found to be composed of three stages. In conclusion, our AC impedance study of CaM is meaningful to reveal the mechanism of CaM in the real life system.
- Calmodulin;Self-assembly;pH value;Ca^2＋;AC impedance
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