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Citation: Kan Jin-Qing, Mu Shao-Lin. The Effect of O-phenanthroline on the Kinetics of the Immobilized Uricase Catalyzed Reaction[J]. Acta Physico-Chimica Sinica, ;1994, 10(07): 648-653. doi: 10.3866/PKU.WHXB19940714 shu

The Effect of O-phenanthroline on the Kinetics of the Immobilized Uricase Catalyzed Reaction

1.
Department of Chemistry, Teachers College, Yangzhou University, Yangzhou 225002

Received Date: 25 February 1993
Available Online: 15 July 1994

The activation energy of the enzyme-catalyzed reaction for uric acid decreases markedly in the presence of o-phenanthroline, which activates the bioelectrochemicla activity of the polypyrrole uricase electrode. The response current of the enzyme electrodeis independent of the concentration of o-phenanthroline. Based on the experimental results, the mechamsm of the enzyme-catalyzed reaction for uric acid in the presence of o-phenanthroline is presented as follows: E+A→EA, EA+S EAS, EAS→EA+P, where E, A, S and P are the enzyme, activator, substrate and product, respectively. The effects of pH value, potential and the uric acid concentration on the response currents of the uricase electrode have been studied in the presence of o-phenanthroline. In the presence of o-phenanthroline, the response current of the enzyme electrode increase linearly with increasing concentration of uric acid in the region of 0.07 to 0.67 mmol·L~(-1), therefore the polypyrrole uricase electrode which has once lost its activity can be activated and used again to determine the substrate concentration.
- Uricase electrode,
- Activator,
- Kinetics of the enzyme-catalyzed reaction
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