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Citation: Tang Houkuan, Xie Changli, Song Zhaohua, Qu Songsheng, Liao Yaoting, Liu Haishui. STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER[J]. Acta Physico-Chimica Sinica, ;1987, 3(02): 113-115. doi: 10.3866/PKU.WHXB19870201 shu

STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER

1.
Department of Chemist; Wuhan University
The First Army Hospital of Guangzhou Military Area

Received Date: 9 July 1986
Available Online: 15 April 1987

In this paper, firstly, we have been studied the multiplication curves of E. coli at exponential phase of growth at 37 ℃ using microcalorimeter, LKB 2277 Bioactivity Monitor, and calculated the multiplication rate constant of E.coli using computer, which is k=0.0397±0.0025 min~(-1). Generation time, G=17.47 minutes, has been obtained from formula G=ln2/k, which is in close agreement with liter- ature value, G=17 minutes. Secondiy, using above method, rate constants of E. coli at different temperatures at exponential of growth have been determined. Finally, according to Arrhenius equation, the activation energy of E. coli multiplication has been obtained, which is 53.09 kJ mol~(-1).
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