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Citation: Di Yang, Jiayi Wei, Hong Zhai, Xin Wang, Taiming Sun, Haole Song, Haiyan Wang. Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”[J]. University Chemistry, ;2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023 shu

Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”

Department of Chemistry and Chemical engineering, Shanxi Datong University, Datong 037009, Shanxi Province, China

Corresponding author: Haiyan Wang, why7135280@126.com
Received Date: 4 December 2023
Revised Date: 4 January 2024

Colloidal gold, a marker widely embraced in commercial point-of-care testing (POCT) for immunochromatographic assays, is distinguished by its vivid color characteristics. It is environmentally benign, morphologically stable, and required no further visual modifications. This study details a straightforward process for fabricating test strips that obviate the need for sophisticated, costly equipment. Colloidal gold, synthesized by reducing the chloroauric acid with trisodium citrate, was conjugated to the nucleocapsid N protein antibody of SARS-CoV-2. With the test strip’s visible area, a competitive antibody-antigen-antibody sandwich complex forms, with the red test line serving as the basis for qualitative analysis of the virus. This detection approach is visually discernible, user-friendly, rapid (within 10 min), sensitive (detection limit of 0.5 ng∙mL⁻¹) and cost-effective (each strip costing no more than 5 yuan). The experimental process offers students an opportunity to engage with the specific biochemical reactions triggered by antigen-antibody interactions, fostering a deep interest in exploring rapid diagnostic technologies that integrate chemistry, biology, life sciences and nanomaterials.
- Colloidal gold,
- Point-of-care testing (POCT),
- Immune chromatography,
- SARS-CoV-2 virus
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