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Citation: Heng Zhang. Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism[J]. University Chemistry, ;2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047 shu

Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism

Heng Zhang ^,

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Corresponding author: Heng Zhang, hengzhang@whu.edu.cn
Received Date: 13 October 2023
Revised Date: 28 November 2023

Two protocols, the transient method and the mathematical method, are introduced to deduce all the rate constants in the enzyme-catalyzed reaction model based on the Michaelis-Menten mechanism. The transient method focuses on analyzing the period before reaching a steady state, while the mathematical method aims to establish a relationship between the concentration of the enzyme and the substrate. By calculating all the rate constants, one can enhance their understanding and grasp of the kinetics of the enzyme-catalyzed reaction.
- Enzyme catalyzed reaction,
- Kinetics,
- Michaelis-Menten mechanism,
- Rate constant
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