Citation: Yuan He, Ye Zhang, Mengchen Wang, Bin Cui, Yan Li, Haiming Fan. Preparation and Kinetic Characterization of Metallo-β-Lactamase L1 in an Online-Offline Blended Teaching Mode[J]. University Chemistry, ;2022, 37(12): 211204. doi: 10.3866/PKU.DXHX202112046 shu

Preparation and Kinetic Characterization of Metallo-β-Lactamase L1 in an Online-Offline Blended Teaching Mode

National Chemistry Experimental Teaching Demonstration Center, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China

Received Date: 17 December 2021

Recombinant protein preparation is an important part of many chemical biology experiments, but its effective implementation in a regular undergraduate experiment course is limited by the long period of time required. This work reports innovative improvements on the content and teaching methods in an undergraduate teaching experiment: “Preparation and kinetic characterization of metallo-β-lactamase L1”. The main improvements include: (1) using online virtual simulation experiments on a mobile device to study the whole process of L1 preparation and conduct a kinetics study; and (2) offline classroom operation for compulsory and extended experiments. The online resource contains 10 modules, such as polymerase chain reaction of a target gene, recombinant plasmid transformation, protein induction, etc. High-quality simulation of experimental scenarios and human-machine interactive operations provide students with a highly immersive experience, and the coherent experimental arrangement presents a clear knowledge framework. A new offline extension experiment was added to enable the use of the isothermal titration microcalorimetric method to characterize the kinetic parameters of the L1 enzyme. This blended teaching mode realizes the integration of online and offline teaching, which can not only improve the quality and efficiency of teaching, but also broaden students’ horizons and cultivate their hands-on ability and innovation awareness. Thus far, good feedback has been received from preliminary implementation, which is expected to provide a reference for undergraduate experimental teaching in other institutions.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
  Majiduddin, F. K.; Materon, I. C.; Palzkill, T. G. Int. J. Med. Microbiol. 2002, 292 (2), 127.
7. [7]
  O’Neill, J. Antimicrobial Resistance: Tackling a crisis for the health and wealth of nations. [2022-03-25]. https://amr-review.org/
8. [8]
  Tooke, C. L.; Hinchliffe, P.; Bragginton, E. C.; Colenso, C. K.; Hirvonen, V. H. A.; Takebayashi, Y.; Spencer, J. J. Mol. Biol. 2019, 431 (18), 3472.
9. [9]
  Coulthurst, S. J.; Barnard, A. M.; Salmond, G. P. Nat. Rev. Microbiol. 2005, 3 (4), 295.
10. [10]
  Wang, W. J.; Wang, Q.; Zhang, Y.; Lu, R.; Zhang, Y. L.; Yang, K. W.; Lei, J. E.; He, Y. Biochim. Biophys. Acta Gen. Subj. 2017, 1861 (8), 2031.
11. [11]
  Zhang, Y.; Lei, J. E.; He, Y.; Yang, J.; Wang, W.; Wasey, A.; Xu, J.; Lin, Y.; Fan, H.; Jing, G.; et al. Angew. Chem. Int. Ed. 2018, 57 (52), 17120.
12. [12]
  Todd, M. J.; Gomez, J. Anal. Biochem. 2001, 296 (2), 179.
1. [1]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
2. [2]
  Zhiguang Xu , Xuan Xu , Qiong Luo , Ganquan Wang , Bin Peng . Reform and Practice of Online and Offline Blended Teaching in Structural Chemistry Course. University Chemistry, 2024, 39(6): 195-200. doi: 10.3866/PKU.DXHX202310112
3. [3]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
4. [4]
  Xia Yin , Peng Yang , Feng Xu , Yan Zhao , Shuangyan Huan , Jingzhe Zhao , Gonglan Ye , Yingpeng Wu . Exploration and Practice of Ideological and Political Education in Inorganic Chemistry Experiment Course. University Chemistry, 2025, 40(7): 119-126. doi: 10.12461/PKU.DXHX202408093
5. [5]
  Shengyan Yang , Xiangzhen Meng , Xin Wang , Yang Zhang . Construction and Exploration of an Online-Offline Blended “Eight-Link” Teaching Method for Physical Chemistry Experiments Based on OBE Concept. University Chemistry, 2024, 39(11): 28-37. doi: 10.3866/PKU.DXHX202402019
6. [6]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044
7. [7]
  Xiaojun Wu , Kai Hu , Faqiong Zhao . Laying the Groundwork for General Chemistry Experiment Teaching: Exploration and Summary of Assisting Experiment Preparatory Work through Online and Offline Integration. University Chemistry, 2024, 39(8): 23-27. doi: 10.3866/PKU.DXHX202312052
8. [8]
  Dexin Tan , Limin Liang , Baoyi Lv , Huiwen Guan , Haicheng Chen , Yanli Wang . Exploring Reverse Teaching Practices in Physical Chemistry Experiment Courses: A Case Study on Chemical Reaction Kinetics. University Chemistry, 2024, 39(11): 79-86. doi: 10.12461/PKU.DXHX202403048
9. [9]
  Ying Yang , Yonghan Wu , Zixuan Li , Lu Zhang , Rongqin Lin , Yefan Zhang , Jiquan Liu , Xiaohui Ning , Yan Li , Bin Cui . Visualization Simulation Experiment of Cyclic Voltammetry (CV) Based on Python. University Chemistry, 2025, 40(10): 233-242. doi: 10.12461/PKU.DXHX202412024
10. [10]
  Wenwen Ma , Liyan Liu , Chengyang Yin , Hongdan Zhang , Lian Kong , Na Wei , Zhan Yu , Zhen Zhao . Exploration of the Online and Offline Mixed Teaching Mode of Specialized English for Chemistry Majors Based on the BOPPPS Model. University Chemistry, 2025, 40(9): 287-294. doi: 10.12461/PKU.DXHX202410026
11. [11]
  Shule Liu . Application of SPC/E Water Model in Molecular Dynamics Teaching Experiments. University Chemistry, 2024, 39(4): 338-342. doi: 10.3866/PKU.DXHX202310029
12. [12]
  Yulian Hu , Xin Zhou , Xiaojun Han . A Virtual Simulation Experiment on the Design and Property Analysis of CO₂ Reduction Photocatalyst. University Chemistry, 2025, 40(3): 30-35. doi: 10.12461/PKU.DXHX202403088
13. [13]
  Linlin Wu , Yonghua Zhou , Zhongbei Li , Liu Deng , Younian Liu , Limiao Chen , Jianhan Huang . Digital Education Promoting Applied Chemistry Comprehensive Experiments: A Case Study of Catalytic Oxidation of Hydrogen Chloride and Reaction Kinetics. University Chemistry, 2025, 40(9): 273-278. doi: 10.12461/PKU.DXHX202411018
14. [14]
  Xuzhen Wang , Xinkui Wang , Dongxu Tian , Wei Liu . Enhancing the Comprehensive Quality and Innovation Abilities of Graduate Students through a “Student-Centered, Dual Integration and Dual Drive” Teaching Model: A Case Study in the Course of Chemical Reaction Kinetics. University Chemistry, 2024, 39(6): 160-165. doi: 10.3866/PKU.DXHX202401074
15. [15]
  Yiying Yang , Dongju Zhang . Elucidating the Concepts of Thermodynamic Control and Kinetic Control in Chemical Reactions through Theoretical Chemistry Calculations: A Computational Chemistry Experiment on the Diels-Alder Reaction. University Chemistry, 2024, 39(3): 327-335. doi: 10.3866/PKU.DXHX202309074
16. [16]
  Huiwen Yao , Ming Yuan , Shunbo Zhang , Yongsheng Gao , Tangbin Liao , Shumin Feng , Li Cao . Practice and Application of the “Online and Offline + BOPPPS” Teaching Model in Organic Chemistry at Traditional Chinese Medicine Institutions. University Chemistry, 2025, 40(11): 127-133. doi: 10.12461/PKU.DXHX202412122
17. [17]
  Hujun Qian , Rui Shi , Guanglu Wu , Xuanbo Zhu . A Preliminary Study on the Development of a Virtual Simulation Platform for Polymer Physics Teaching and Its Teaching Practice. University Chemistry, 2025, 40(4): 147-153. doi: 10.12461/PKU.DXHX202409009
18. [18]
  Pingping Zhu , Yongjun Xie , Yuanping Yi , Yu Huang , Qiang Zhou , Shiyan Xiao , Haiyang Yang , Pingsheng He . Excavation and Extraction of Ideological and Political Elements for the Virtual Simulation Experiments at Molecular Level: Taking the Project “the Simulation and Computation of Conformation, Morphology and Dimensions of Polymer Chains” as an Example. University Chemistry, 2024, 39(2): 83-88. doi: 10.3866/PKU.DXHX202309063
19. [19]
  Wenwen Zhang , Peichao Zhang , Conghao Gai , Xiaoyun Chai , Yan Zou , Qingjie Zhao . Unveiling Kinetics at Natural Abundance: ¹³C NMR Isotope Effect Experiments. University Chemistry, 2025, 40(10): 203-207. doi: 10.12461/PKU.DXHX202411076
20. [20]
  Jinfu Ma , Hui Lu , Jiandong Wu , Zhongli Zou . Teaching Design of Electrochemical Principles Course Based on “Cognitive Laws”: Kinetics of Electron Transfer Steps. University Chemistry, 2024, 39(3): 174-177. doi: 10.3866/PKU.DXHX202309052

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(891)
HTML views(123)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142