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Citation: GAN Ning, YANG Qian. Comprehensive Analytical Chemistry Experiment on MicroRNA Fluorescence Sensor Based on Two Dimensional MOFs Probes and New Teaching Mode of “Game-Study Combination and Trinity Evaluation”[J]. University Chemistry, ;2019, 34(6): 38-45. doi: 10.3866/PKU.DXHX201810003 shu

Comprehensive Analytical Chemistry Experiment on MicroRNA Fluorescence Sensor Based on Two Dimensional MOFs Probes and New Teaching Mode of “Game-Study Combination and Trinity Evaluation”

GAN Ning ,
YANG Qian

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, Zhejiang Province, P. R. China

Received Date: 8 October 2018

This comprehensive analytical experiment has successfully introduced the frontier of science such as two-dimensional metal organic frameworks (2D MOFs) fabrication and MicroRNA (miRNA) analysis into laboratory teaching for undergraduate and graduate students whose major are chemistry or materials science. It mainly includes the following issues:synthesis and characterization of 2D MOFs, construction of 2D MOFs-DNA fluorescent probe, and developing the miRNA fluorescence biosensor. The experiment takes 10 hours. In the experiment, we adopted a new teaching mode of "game-study combination and trinity evaluation". The students learned a breadth of experimental skills, improved their experimental skills and broadened their academic vision. Moreover, through the combination of competition and teaching, the students' study enthusiasms were greatly stimulated, the spirit of teamwork was cultivated. This experiment and the teaching mode are also suitable for promotion to other comprehensive chemistry laboratory teaching.
- Comprehensive analytical chemistry experiment,
- Fluorescence sensor,
- Two-dimensional metal organic frameworks (2D MOFs),
- MicroRNA,
- Game-study combination,
- Trinity evaluation
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