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Citation: ZHAO Huinan, ZHANG Yanxia, XUE Xia, DAI Kun, ZHENG Wenjing, MA Cheng, ZHU Jianhua, LIU Yanming, ZHANG Feng. Analysis of nine antioxidants in vegetable oils by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2020, 38(7): 841-846. doi: 10.3724/SP.J.1123.2019.11023 shu

Analysis of nine antioxidants in vegetable oils by high performance liquid chromatography

ZHAO Huinan ¹ ,
ZHANG Yanxia ¹ ,
XUE Xia ¹ ,
DAI Kun ¹ ,
ZHENG Wenjing ¹ ,
MA Cheng ¹ ,
ZHU Jianhua ¹ ,
LIU Yanming ^1,, ,
ZHANG Feng ^2,,

1.
Shandong Institute for Food and Drug Control, Ji'nan 250101, China;
2.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China

Corresponding author: LIU Yanming, ZHANG Feng,
Received Date: 26 November 2019

Fund Project: Shandong Key Point Research and Invention Program (Food for Special Medical Purpose, FSMP) (No. 2019YYSP020)

A method was developed for the determination of nine antioxidants in vegetable oils by high performance liquid chromatography (HPLC). The samples were extracted with methanol, and the fat in the samples was degreased by freezing. Separation of the targeted compounds was performed on an XBridge C₁₈ column in gradient elution mode using methanol-0.1% (v/v) formic acid aqueous solution as the mobile phase. The analytes were detected using a diode-array detector by the external standard method. The stability and storage conditions for the nine antioxidants were systematically investigated. Ascorbyl palmitate (AP) was introduced into the preparation and pre-treatment of the targets. The concentration of AP was optimized to improve the stability and recovery of the targets. The effects of different extraction solvents and purification methods on the extraction efficiencies were discussed. The results showed that the nine antioxidants could be separated well under the optimized conditions. Good linear relationships in the linear range were obtained, and the correlation coefficients (R²) were greater than 0.999. The average recoveries of the nine antioxidants ranged from 85.3% to 104.1%, with RSDs of the method ≤5.0%. The limits of quantitation (LOQs) for the nine synthetic antioxidants were in the range of 0.6-3.0 mg/kg. The method is simple, rapid, sensitive, and it shows good recovery and reproducibility.
- high performance liquid chromatography (HPLC),
- ascorbyl palmitate (AP),
- antioxidants,
- vegetable oils,
- stability
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