Citation: HU Yechen, JIANG Bo, ZHANG Lihua, ZHANG Yukui. Advancesin enrichment and detection methods for N-phosphorylated proteins[J]. Chinese Journal of Chromatography, ;2020, 38(3): 278-286. doi: 10.3724/SP.J.1123.2019.09015 shu

Advancesin enrichment and detection methods for N-phosphorylated proteins

HU Yechen ^1,2 ,
JIANG Bo ¹ ,
ZHANG Lihua ^1,, ,
ZHANG Yukui ¹

1.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: ZHANG Lihua, lihuazhang@dicp.ac.cn
Received Date: 12 September 2019

Fund Project: National Natural Science Foundation of China (Nos. 21505133, 91753110).

Protein phosphorylation plays an important role in various life processes, such as signaling, metabolism, and growth. In addition to the well-known O-phosphorylation, which occurs on serine, threonine and tyrosine side chains, N-phosphorylation, occurring on histidine, arginine and lysine side chains, has attracted much attention. However, due to the chemical instability of N-phosphorylation, the studies on it are still in the nascent stage. In this review, enrichment and detection methods for protein N-phosphorylation are reviewed, and future research in this field is prospected.
- protein N-phosphorylation,
- enrichment,
- detection,
- review
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