Citation: YANG Jina, LIU Danyang, ZHOU Ting. Recent advances in sample preparation techniques for the lipid profiling based on chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(1): 74-85. doi: 10.3724/SP.J.1123.2019.06023 shu

Recent advances in sample preparation techniques for the lipid profiling based on chromatography-mass spectrometry

School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China

Corresponding author: ZHOU Ting, tingzhou@scut.edu.cn
Received Date: 25 June 2019

Fund Project: Supported by National Natural Science Foundation of China (No. 21775047) Pearl River S&T Nova Program of Guangzhou, China (No. 201806010055) Fundamental Research Funds for the Central Universities (No. 2018MS55).

As the main structural components of cellular and sub-cellular membranes, lipids are a major source of energy and play an important role in various biological processes such as cellular signaling. Lipid profiling has attracted increasing attention in recent years, and chromatography-mass spectrometry techniques for lipid profiling occupy a dominant position in this regard. Sample preparation, which aims at enriching trace substances and reducing matrix interferences, is a crucial step in the analysis of lipids for the intricacy of sample matrices. Here, we review recent developments in sample preparation techniques based on chromatography-mass spectrometry and their application to lipid profiling. Various sample preparation techniques are described and summarized. The extraction methods based on liquid phase included liquid-liquid extraction and single organic solvent extraction. The extraction methods based on solid phase involved solid-phase extraction and solid-phase microextraction. The field-assisted extraction methods cover supercritical fluid extraction, pressurized liquid extraction, microwave-assisted extraction, and ultrasound-assisted extraction. Moreover, online coupling sample preparation methods and sample preparation methods for in vivo lipid analysis are presented. Finally, the problems and trends in sample preparation techniques for lipid profiling based on chromatography-mass spectrometry are discussed. It is believed that efficient development of sample preparation techniques would help improve the sensitivity and selectivity of lipid profiling as well as the analysis speed.
- sample preparation,
- lipid profiling,
- chromatography-mass spectrometry
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