Citation: GAO Jie, WU Qi, CHEN Lixiao, LI Hui, DONG Shuqing, LUO Guoying, ZHANG Shusheng, QIU Hongdeng, ZHAO Liang. Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C₁₈[J]. Chinese Journal of Chromatography, ;2020, 38(4): 414-423. doi: 10.3724/SP.J.1123.2019.03009 shu

Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C₁₈

GAO Jie ^1,2 ,
WU Qi ^1,2 ,
CHEN Lixiao ^1,2 ,
LI Hui ¹ ,
DONG Shuqing ¹ ,
LUO Guoying ¹ ,
ZHANG Shusheng ³ ,
QIU Hongdeng ^1,, ,
ZHAO Liang ^1,,

1.
Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China

Corresponding author: QIU Hongdeng, ZHAO Liang,
Received Date: 11 March 2019

Fund Project: National Natural Science Foundation of China (Nos. 21702210, 21675163).

A novel reversed/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose (DMCC)-functionalized C₁₈ was prepared by covalent bonding between the amino groups of octadecylamine with the aldehyde groups of DMCC, which was used in reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) modes. The prepared DMCC-functionalized C₁₈ modified silica (C₁₈-DMCC/SiO₂) stationary phase exhibited good hydrophobic selectivity and aromatic selectivity by separating alkylbenzenes and polycyclic aromatic hydrocarbons (PAHs) in the RPLC mode. Polar compounds, including anilines, phenols, and glycosides were chosen as analytes to evaluate the polar selectivity of this column in the RPLC mode, and the evaluation results were satisfactory compared with the commercial C₁₈ column. Nucleobases were used for evaluating the hydrophilic interaction liquid chromatography performance of the C₁₈-DMCC/SiO₂ column. By investigating the impact of organic solvent content on the retention, it could be found that this new stationary phase had the typical characteristics of reversed-phase/hydrophilic chromatography.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
1. [1]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
2. [2]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
3. [3]
  Qiuping Liu , Yongxian Fan , Wenxian Chen , Mengdi Wang , Mei Mei , Genrong Qiang . Design of Ideological and Political Education for the Preparation Experiment of Ferrous Sulfate. University Chemistry, 2024, 39(2): 116-120. doi: 10.3866/PKU.DXHX202309083
4. [4]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
5. [5]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
6. [6]
  Yufan Pan , Xue Ding , Jiayu Lin , Haiting Wu , Hairong Huang , Cuixue Chen , Meiling Ye . Oil Cosmetics, Charming Chemistry: A Gradient Science Popularization Scheme for Cream Cosmetic Preparation. University Chemistry, 2025, 40(4): 382-389. doi: 10.12461/PKU.DXHX202406078
7. [7]
  Xinzhe HUANG , Lihui XU , Yue YANG , Liming WANG , Zhangyong LIU , Zhongjian WANG . Preparation and visible light responsive photocatalytic properties of BiSbO₄/BiOBr. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 284-292. doi: 10.11862/CJIC.20240212
8. [8]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
9. [9]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
10. [10]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
11. [11]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
12. [12]
  Jiaxin Su , Jiaqi Zhang , Shuming Chai , Yankun Wang , Sibo Wang , Yuanxing Fang . Optimizing Poly(heptazine imide) Photoanodes Using Binary Molten Salt Synthesis for Water Oxidation Reaction. Acta Physico-Chimica Sinica, 2024, 40(12): 2408012-. doi: 10.3866/PKU.WHXB202408012
13. [13]
  Xiaoning TANG , Junnan LIU , Xingfu YANG , Jie LEI , Qiuyang LUO , Shu XIA , An XUE . Effect of sodium alginate-sodium carboxymethylcellulose gel layer on the stability of Zn anodes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1452-1460. doi: 10.11862/CJIC.20240191
14. [14]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
15. [15]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
16. [16]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
17. [17]
  Heng Chen , Longhui Nie , Kai Xu , Yiqiong Yang , Caihong Fang . 两步焙烧法制备大比表面积和结晶性增强超薄g-C₃N₄纳米片及其高效光催化产H₂O₂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406019-. doi: 10.3866/PKU.WHXB202406019
18. [18]
  Haoxiang Zhang , Zhihan Zhao , Yongchen Jin , Zhiqiang Niu , Jinlei Tian . Synthesis of an Efficient Absorbent Gel: A Recommended Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(11): 251-258. doi: 10.12461/PKU.DXHX202401084
19. [19]
  Shijie Li , Ke Rong , Xiaoqin Wang , Chuqi Shen , Fang Yang , Qinghong Zhang . Design of Carbon Quantum Dots/CdS/Ta₃N₅ S-Scheme Heterojunction Nanofibers for Efficient Photocatalytic Antibiotic Removal. Acta Physico-Chimica Sinica, 2024, 40(12): 2403005-. doi: 10.3866/PKU.WHXB202403005
20. [20]
  Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101

Get Citation

PDF

XML

Metrics

PDF Downloads(6)
Abstract views(312)
HTML views(78)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C18

Metrics

通讯作者: 陈斌, bchen63@163.com

Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C₁₈