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Citation: SHEN Huilian, ALIMU Kahaer. Selective enrichment of phosphopeptides with aspartic acid based immobilized metal ion affinity chromatography materials[J]. Chinese Journal of Chromatography, ;2018, 36(4): 334-338. doi: 10.3724/SP.J.1123.2017.10007 shu

Selective enrichment of phosphopeptides with aspartic acid based immobilized metal ion affinity chromatography materials

SHEN Huilian ^1, ,
ALIMU Kahaer ²

1.
Liaoning National Normal College, Shenyang 110032, China;
2.
Xinjiang Agricultural Vocational Technical College, Changji 831100, China

Received Date: 20 October 2017

A natural amino acid, aspartic acid, was modified to bind to silica microspheres with a click chemistry method (-Click Asp).The modified Click Asp microspheres were further modified by incorporating Fe³⁺ ions, resulting in Fe³⁺ and Click Asp based immobilized ion affinity chromatography (IMAC)(Fe³⁺-Click Asp).The prepared materials were characterized using Fourier transform infrared spectrometry, X-ray photoelectron spectrometry, and scanning electron microscopy, and it was confirmed that Fe³⁺-Click Asp was successfully prepared.Subsequently, the Fe³⁺-Click Asp microspheres were used to enrich phosphopeptides from tryptic digests of proteins and milk.It was found that the phosphopeptides could be selectively enriched with this material.This study describes a novel material and enrichment method for phosphoproteomics.
- aspartic acid,
- solid phase extraction (SPE),
- immobilized metal ion affinity chromatography (IMAC),
- phosphopeptide,
- enrichment
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