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Citation: NIU Lixi, MA Shishi. Analysis of 2, 3-pyridinedicarboximide and its products conversed by engineering strain with high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2018, 36(4): 413-415. doi: 10.3724/SP.J.1123.2017.09039 shu

Analysis of 2, 3-pyridinedicarboximide and its products conversed by engineering strain with high performance liquid chromatography

NIU Lixi ,
MA Shishi

Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China

Received Date: 9 October 2017

A high performance liquid chromatography (HPLC) method was established for the detection of 2, 3-pyridinedicarboximide (PDI) and its enzyme reaction products, 3-carbamoyl-α-picolinic acid (α-3CP), using an engineering strain containing the D-hydantoinase gene expression box.The strain pET3a-hyd/BL21(DE3) was collected after induction and added to a PDI saturated aqueous solution.After reacting at 37℃ for 30 min with constant stirring, the supernatant was separated by centrifugation at 13000 r/min and detected by HPLC.The chromatographic conditions were as follows:Hypersil^TM GOLD C18 column (250 mm×4.6 mm, 5 μm), H₂O-acetonitrile (90:10, v/v) containing 0.1%(v/v) trifluoroacetic acid as the mobile phase with a flow rate of 1 mL/min and a detection wavelength of 254 nm.The specific activity of pET3a-hyd/BL21(DE3) was found to be 0.61 U/(mL·10OD_{600 nm}).This study provides a theoretical basis for the preparation of complicated half-amides using biological methods.
- high performance liquid chromatography (HPLC),
- 3-carbamoyl-α-picolinic acid,
- 2,3-pyridinedicarboximide,
- engineering strain
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