Citation: JIA Youmei, CAI Jianfeng, XIN Huaxia, FENG Jiatao, FU Yanhui, FU Qing, JIN Yu. Separation and purification of the components in Trachelospermum jasminoides by two dimensional hydrophilic interaction liquid chromatography- reversed-phase liquid chromatography[J]. Chinese Journal of Chromatography, ;2017, 35(6): 650-655. doi: 10.3724/SP.J.1123.2017.01010 shu

Separation and purification of the components in Trachelospermum jasminoides by two dimensional hydrophilic interaction liquid chromatography- reversed-phase liquid chromatography

JIA Youmei ^1, ,
CAI Jianfeng ¹ ,
XIN Huaxia ¹ ,
FENG Jiatao ² ,
FU Yanhui ³ ,
FU Qing ¹ ,
JIN Yu ¹

1.
School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
2.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
3.
College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China

Received Date: 11 January 2017

Fund Project: National Natural Science Foundation of China (No. 81403100).

A preparative two dimensional hydrophilic interaction liquid chromatography/reversed-phase liquid chromatography (Pre-2D-HILIC/RPLC) method was established to separate and purify the components in Trachelospermum jasminoides. The pigments and strongly polar components were removed from the crude extract after the active carbon decolorization and solid phase extraction processes. A Click XIon column (250 mm×20 mm, 10 μm) was selected as stationary phase and water-acetonitrile as mobile phases in the first dimensional HILIC. Finally, 15 fractions were collected under UV-triggered mode. In the second dimensional RPLC, a C18 column (250 mm×20 mm, 5 μm) was selected and water-acetonitrile was used as mobile phases. As a result, 14 compounds with high purity were obtained, which were further identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR). Finally, 11 lignan compounds and three flavonoid compounds were obtained. The method has a good orthogonality, and can improve the resolution and the peak capacity. It is significant for the separation of complex components from Trachelospermum jasminoides.
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Separation and purification of the components in Trachelospermum jasminoides by two dimensional hydrophilic interaction liquid chromatography- reversed-phase liquid chromatography

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com