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Citation: YAN Lijuan, LI Wenbin, HONG Yuchen, WU Min, XU Dunming, LIN Liyi, GU Chunyan. Determination of vitamin D in infant formula using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(4): 427-431. doi: 10.3724/SP.J.1123.2016.10036 shu

Determination of vitamin D in infant formula using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry

YAN Lijuan ^1, ,
LI Wenbin ² ,
HONG Yuchen ¹ ,
WU Min ¹ ,
XU Dunming ¹ ,
LIN Liyi ¹ ,
GU Chunyan ²

1.
Technology Center of Xiamen Entry-exit Inspection and Quarantine Bureau, Xiamen 361026, China;
2.
Beijing Dikma Technologies Inc., Beijing 100029, China

Received Date: 12 October 2016

Fund Project: Natural Science Foundation of Fujian Province (Nos. 2016J01071, 2014J01057)

A method using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS) was developed for the determination of vitamin D in infant formula. The milk powder sample was extracted with hexane and tert-butyl methyl ether followed by clean-up with ProElut VDC cartridge. The LC separation was performed on Kinetex C₁₈ column (100 mm×4.6 mm, 2.6 μm) by a gradient elution using water and acetonitrile as the mobile phases with a flow rate of 1.0 mL/min. Identification of vitamin D₂ and vitamin D₃ was achieved by APCI-MS/MS using multiple reaction monitoring (MRM). The quantification analysis was performed by internal standard method. The calibration curves showed a good linearity in the range of 5-5000 μg/L. The limits of detection (LODs) for vitamin D₂ and vitamin D₃ in the formula were 2 μg/kg. The limits of quantification (LOQs) for vitamin D₂ and vitamin D₃ in the formula were 5 μg/kg. The average recoveries of vitamin D from milk powder spiked at 5, 10 and 100 μg/kg levels were between 85.2% and 105.3%, and the relative standard deviations were between 4.7% and 8.1%. The simple, accurate and sensitive method is suitable for the determination of vitamin D in infant formula.
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