Citation: WENG Zhongya, XUE Yun, SHI Wenjun, WANG Yan, YAN Chao. Preparation of a new organic-silica hybrid monolithic column and its applications in capillary liquid chromatography and pressurized capillary electrochromatography[J]. Chinese Journal of Chromatography, ;2016, 34(5): 467-472. doi: 10.3724/SP.J.1123.2015.12040 shu

Preparation of a new organic-silica hybrid monolithic column and its applications in capillary liquid chromatography and pressurized capillary electrochromatography

1.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: WANG Yan, YAN Chao,
Received Date: 1 January 2016

Fund Project: 国家自然科学基金项目(21175092) (21175092)上海市科委科研计划项目(15142200200) (15142200200)上海市教育委员会科研创新项目(14YZ170) (14YZ170)中国博士后科学基金项目(2015M581628). (2015M581628)

The organic-silica hybrid poly(POSS-co-AM) monolithic columns were prepared by in-situ polymerization process with octavinyl-T8-silsesquioxane (POSS) as a crosslinking agent and acrylamide (AM) as monomer, tetrahydrofuran (THF) as a porogenic agent and azobisisobutyronitrile (AIBN) as initiator. In order to optimize the properties, the composition of the polymerization mixture was investigated. The optimum preparation conditions were as follows: both the mass ratios of monomer to porogen and POSS to AM were 1.0: 5.0; the mass fraction of the initiator (AIBN) was 0.1%. With the help of POSS materials, homogeneous column bed and good permeability were obtained. The columns were used in capillary liquid chromatography (cLC) and pressurized capillary electrochromatography (pCEC) for the separation of mixtures of amines, nucleosides, nitrobenzene amines. Satisfactory separations for these samples are achieved. The column is proved to be useful in both reversed-phase chromatography (RPLC) and hydrophilic interaction chromatography (HILIC).
1. [1]
  [1] Zhang Z B, Ou J J, Dong J, et al. Chinese Journal of Chromatography, 2011, 29(9): 830 张振宾, 欧俊杰, 董靖, 等. 色谱, 2011, 29(9): 830
2. [2]
  [2] Yan L J, Zhang Q H, Li T, et al. Chinese Journal of Chromatography, 2005, 23(5): 499 严丽娟, 张庆合, 李彤, 等. 色谱, 2005, 23(5): 499
3. [3]
  [3] Zhang Z B, Ou J J, Lin H, et al. Chemical Journal of Chinese Universities, 2013, 34(9): 2011 张振宾, 欧俊杰, 林辉, 等. 高等学校化学学报, 2013, 34(9): 2011
4. [4]
  [4] Alves F, Nischang I. Chem Eur J, 2013, 19(51): 17310
5. [5]
  [5] Ou J J, Zhang Z B, Lin H, et al. Anal Chim Acta, 2013, 761: 209
6. [6]
  [6] Kannan R Y, Salacinski H J, Butler P E, et al. Acc Chem Res, 2005, 38: 879
7. [7]
  [7] Wang K Y, Chen Y Z, Yang H H, et al. Talanta, 2012, 91(6): 52
8. [8]
  [8] Lin X C, Wang X, Zhao T T, et al. J Chromatogr A, 2012, 1260: 174
9. [9]
  [9] Zhang X Y, Yang G L. China Journal of Pharmaceutical Economics, 2013(1): 235 张晓燕, 杨更亮. 中国药物经济学, 2013(1): 235
10. [10]
  [10] Kuang Y Y, Wang Y, Gu X, et al. Chinese Journal of Chromatography, 2014, 32(4): 388 况媛媛, 王彦, 谷雪, 等. 色谱, 2014, 32(4): 388
11. [11]
  [11] Bristow P A, Knox J H. Chromatographia, 1977, 10(6): 279
12. [12]
  [12] Lide D R. The CRC Handbook of Chemistry and Physics. 80th ed. S. I.: CRC Press, 1999
13. [13]
  [13] Gao Y, Wang Y, Wang C R, et al. Chinese Journal of Chromatography, 2012, 30(5): 487 高也, 王彦, 王超然, 等. 色谱, 2012, 30(5): 487
14. [14]
  [14] Karatapanis A E, Fiamegos Y C, Stalikas C D. J Chromatogr A, 2011, 1218: 2871
15. [15]
  [15] Dou A B, Zou C J, Shan Y H, et al. Chinese Journal of Chromatography, 2014, 32(5): 447 窦阿波, 邹存杰, 单圆鸿, 等. 色谱, 2014, 32(5): 447
16. [16]
  [16] Lin J, Huang G H, Lin X C, et al. Electrophoresis, 2008, 29(19): 4055
17. [17]
  [17] Xu L, Lee H K. J Chromatogr A, 2008, 1195: 78
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沈阳化工大学材料科学与工程学院沈阳 110142