Citation: QI Weimei, ZHAO Xian-en, QI Yong, SUN Zhiwei, CHEN Guang, YOU Jinmao, SUO Yourui. Determination of L-dopa and dopamine in rat brain microdialysate by ultra high performance liquid chromatography-tandem mass spectrometry using stable isotope-coded derivatization coupled with dispersive liquid-liquid microextraction[J]. Chinese Journal of Chromatography, ;2015, 33(9): 981-987. doi: 10.3724/SP.J.1123.2015.04049 shu

Determination of L-dopa and dopamine in rat brain microdialysate by ultra high performance liquid chromatography-tandem mass spectrometry using stable isotope-coded derivatization coupled with dispersive liquid-liquid microextraction

QI Weimei ¹ ,
ZHAO Xian-en ^2,, ,
QI Yong ¹ ,
SUN Zhiwei ² ,
CHEN Guang ² ,
YOU Jinmao ^2,3,, ,
SUO Yourui ³

1.
1. Chemical Department, Laiwu Vocational and Technical College, Laiwu 271100, China;

Corresponding author: ZHAO Xian-en, YOU Jinmao,
Received Date: 28 April 2015

Fund Project: 国家自然科学基金项目(81303179). (81303179)

The sensitive detection method of levodopa (L-DOPA) and dopamine (DA) in rat brain microdialysate of Parkinson's disease (PD) is an essential tool for the clinical study and attenuated synergistic drug screening for L-DOPA from traditional Chinese medicines. Using d₀/d₃-10-methyl-acridone-2-sulfonyl chloride (d₀/d₃-MASC) as stable isotope derivatization reagent, a novel ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for L-DOPA and DA by stable isotope-coded derivatization coupled with ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME). d₀-MASC (light) and d₃-MASC (heavy) were used as derivatization reagents for microdialysate samples and standards, respectively. Mixtures of the two solutions were prepared by UA-DLLME for UHPLC-MS/MS analysis with multiple reaction monitoring (MRM) mode. With d₃-MASC heavy derivatives as internal standards for corresponding light derivatives from samples, the stable isotope internal standard quantification for L-DOPA and DA was carried out. The stable derivatives were obtained in aqueous acetonitrile (pH 10.8 sodium carbonate-sodium bicarbonate buffer) at 37 ℃ for 3.0 min, and then were separated within 2.0 min using gradient elution. Linear range was 0.20-1500.0 nmol/L (R> 0.994). LODs were 0.005 and 0.009 nmol/L for DA and L-DOPA (S/N=3), respectively. This method was validated, and it showed obvious advantages in comparing with the reported methods in terms of sensitivity, analysis speed and anti-matrix interference. This method has been successfully applied to the study of effect of Shouwu Fang on L-DOPA and DA concentration fluctuations in PD rat brain microdialysate.
1. [1]
  [1] Zhou H H. Pharmacology. Beijing: Science Press (周宏灏. 药理学. 北京: 科学出版社), 2007: 115
2. [2]
  [2] Wang D Q, Wang W, Jing F C, et al. Chinese Pharmacological Bulletin (王丹巧, 王巍, 景富春, 等. 中国药理学通报), 2007, 23(11): 1527
3. [3]
  [3] Sun X F, Wang D Q, Wu Z E, et al. Chinese Journal of Experimental Traditional Medical Formulae (孙晓芳, 王丹巧, 吴兆恩, 等. 中国实验方剂学杂志), 2011, 17(11): 111
4. [4]
  [4] Perry M, Li Q, Kennedy R T. Anal Chim Acta, 2009, 653: 1
5. [5]
  [5] Zhao Y Y, Liu L Y, Han Y Y, et al. Chinese Journal of Chromatography (赵燕燕, 刘丽艳, 韩媛媛, 等. 色谱), 2011, 29(2): 146
6. [6]
  [6] Song Y Q, Li F Y, Li W, et al. Chinese Journal of Health Laboratory Technology (宋彦强, 李凤云, 李玮, 等. 中国卫生检验杂志), 2013, 23(5): 1339
7. [7]
  [7] Song P, Mabrouk O S, Hershey N D, et al. Anal Chem, 2012, 84: 412
8. [8]
  [8] Zhao X E, Suo Y R. Talanta, 2008, 76: 690
9. [9]
  [9] Zhao X E, Suo Y R. Chinese Journal of Analytical Chemistry (赵先恩, 索有瑞. 分析化学), 2008, 36(1): 12
10. [10]
  [10] Ji C J, Li W L, Ren X D, et al. Anal Chem, 2008, 80: 9195
11. [11]
  [11] Liu P, Huang Y Q, Cai W J, et al. Anal Chem, 2014, 86: 9765
12. [12]
  [12] Leng J P, Wang H Y, Zhang L, et al. Anal Chim Acta, 2013, 758: 114
13. [13]
  [13] Zhang S J, You J M, Ning S J, et al. J Chromatogr A, 2013, 1280: 84
14. [14]
  [14] Dai W D, Huang Q, Yin P Y, et al. Anal Chem, 2012, 84: 10245
15. [15]
  [15] Sun Z W, Wang X X, Cai Y P, et al. Talanta, 2014, 120: 84
16. [16]
  [16] Yuan K, Kang H N, Yue Z F, et al. Anal Chim Acta, 2015, 866: 41
17. [17]
  [17] Zhao X E, Lü T, Wei N, et al. Chinese Journal of Analytical Chemistry (赵先恩, 吕涛, 魏娜, 等. 分析化学), 2014, 42(11): 1629
18. [18]
  [18] Wang X Y, Qi W M, Zhao X E, et al. Chinese Journal of Chromatography (王晓燕, 亓伟梅, 赵先恩, 等. 色谱), 2014, 32(6): 623
19. [19]
  [19] Wu C Q, Lei J M, Li Y L, et al. Chinese Journal of Chromatography (吴翠琴, 雷金妹, 李韵灵, 等. 色谱), 2014, 32(12): 1362
20. [20]
  [20] Wu Z E, Niu X H, Zhang M Y, et al. Chinese Journal of Pharmaceutical Analysis (吴兆恩, 牛晓红, 张美玉, 等. 药物分析杂志), 2012, 32(2): 217
21. [21]
  [21] Tufi S, Lamoree M, Boer J D, et al. J Chromatogr A, 2015, 1395: 79
22. [22]
  [22] Junnotula V, Licea-Perez H. J Chromatogr B, 2013, 926: 47
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