Citation: XU Ying, ZANG Ying, JIANG Ting, ZHENG Zhaojuan, OUYANG Jia. Simultaneous analysis of trehalose, glucose and maltose in biotransformation samples by high performance anion exchange chromatography with pulsed ampere detection[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1400-1403. doi: 10.3724/SP.J.1123.2014.07037 shu

Simultaneous analysis of trehalose, glucose and maltose in biotransformation samples by high performance anion exchange chromatography with pulsed ampere detection

1.
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

Corresponding author: OUYANG Jia,
Received Date: 23 July 2014
Available Online: 8 September 2014
Fund Project: 国家自然科学基金项目(31200443) (31200443)江苏省杰出青年基金项目(BK2012038) (BK2012038)新世纪优秀人才支持计划项目(NCET-11-0988) (NCET-11-0988)

An analytical method for the determination of trehalose, maltose, and glucose in biotransformation samples was developed by using high performance anion exchange chromatography coupled with pulsed ampere detection (HPAEC-PAD). The analysis was performed on a CarboPac^TM10 column (250 mm×2 mm) with the gradient elution of NaOH-NaAc as the mobile phase. The column temperature was set at 30 ℃, the flow rate was 0.30 mL/min. The results showed that trehalose, maltose, and glucose in biotransformation system were completely separated and determined in 15 min. The linear ranges and the working curves were determined by using standard samples. The correlation coefficients of three kinds of carbohydrates were over 0.9998 . The detection limits (LODs) were 0.010-0.100 mg/L. Under the optimized separation conditions, the recoveries of saccharides in the transformation system at three different spiked levels ranged from 89.4% to 103.2%. In biotransformation system, 50 IU trehalose synthase were added into 200 g/L maltose for reaction of 8 h at 37 ℃, pH 8.0. Under the above conditions, the concentration of trehalose in biotransformation sample was 101.084 g/L, and the conversion rate of trehalose reached 50.5%. The method can be applied to determine the composition in the transformation system with the advantages of simplicity and convenience.
- high performance anion exchange chromatography (HPAEC),
- trehalose,
- glucose,
- maltose,
- biotransformation sample
1. [1]
  [1] Schiraldi C, Di Lernia I, De Rosa M. Trends Biotechnol, 2002, 20(10): 420
2. [2]
  [2] Li Q, Yuan Q S. Chinese Journal of Biochemical Pharmaceutics (李群, 袁勤生. 中国生化药物杂志), 1995, 16(5): 231
3. [3]
  [3] Wu X L, Yue M, Ding H B. Microbiology China (吴秀丽, 岳明, 丁宏标. 微生物学通报), 2009, 36(7): 1067
4. [4]
  [4] Miao J. Microbiology China (缪静. 微生物学通报), 2004, 31(5): 96
5. [5]
  [5] Zhou J, Liu J H, Ouyang X L, et al. Journal of Experimental Hematology (周俊, 刘景汉, 欧阳锡林, 等. 中国实验血液学杂志), 2004, 12(6): 837
6. [6]
  [6] Wang L, Xiao D G, Zhang Z. Industrial Microbiology (王兰, 肖冬光, 张正. 工业微生物), 2002, 32(2): 18
7. [7]
  [7] Wang L, Xiao D G. Biotechnology (王兰, 肖冬光. 生物技术), 2002, 12(3): 27
8. [8]
  [8] Zhao H, He C F, Qin Y R, et al. Journal of Jishou University: Natural Science Edition (赵华, 何聪芬, 秦允荣, 等. 吉首大学学报: 自然科学版), 2009, 30(1): 79
9. [9]
  [9] Mou S F, Yu H, Cai Y Q. Chinese Journal of Chromatography (牟世芬, 于泓, 蔡亚岐. 色谱), 2009, 27(5): 667
10. [10]
  [10] Yu H, Ding Y S, Mou S F, et al. J Chromatogr A, 2002, 966(1/2): 89
11. [11]
  [11] Li J, Li R Y, Liang L N. Chinese Journal of Analytical Chemistry (李静, 李仁勇, 梁立娜. 分析化学), 2012, 40(9): 1415
12. [12]
  [12] Zhang L, Zhou G M, Xiong J F. Food Science (张磊, 周光明, 熊建飞. 食品科学), 2012, 33(8): 159
13. [13]
  [13] Zhou H B, Xiong Z Y, Li P, et al. Chinese Journal of Chromatography (周洪斌, 熊治渝, 李平, 等. 色谱), 2013, 31(11): 1093
14. [14]
  [14] Pan G W, Liu L Y, Ye M L, et al. Chinese Journal of Chromatography (潘广文, 刘绿叶, 叶明立, 等. 色谱), 2012, 30(4): 395
15. [15]
  [15] Ding H L, Li C, Jin P, et al. Chinese Journal of Chromatography (丁洪流, 李灿, 金萍, 等. 色谱), 2013, 31(8): 804
16. [16]
  [16] Wei Y, Ding M Y. Chinese Journal of Chromatography (魏泱, 丁明玉. 色谱), 2001, 19(3): 226
17. [17]
  [17] Xu L, Zhou G M, Yu N. China Surfactant Detergent & Cosmetics (许丽, 周光明, 余娜, 等. 日用化学工业), 2012, 42(1): 77
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