Citation: XIE Hanbing, ZHOU Mingying, ZHAO Haifeng, WANG Yigang, JIANG Wanfeng, ZHAO Shan. Determination of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(5): 493-498. doi: 10.3724/SP.J.1123.2013.12029 shu

Determination of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry

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1. Qingdao Quality Supervision and Inspection Center for Shopping Basket Commodity, Qingdao 266071, China;

Corresponding author: ZHOU Mingying,
Received Date: 25 December 2013
Available Online: 26 February 2014
Fund Project: 青岛市2013年“菜篮子”工程建设资助项目(青菜篮子[2013]1号). (青菜篮子[2013]1号)

This study was aimed to the establishment of an analytical method for the determination of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS). The target compounds were gibberellins, 6-benzylaminopurine and parachlorophenoxyacetic acid. The QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was used for sample preparation. The analytes were extracted with a solution containing 1% (v/v, if not specified) acetic, 50% ethanol, 49% acetonitrile, and cleaned-up by dispersive solid-phase extraction with diatomite dispersant, then degreased by hexane. The three target compounds were separated on an Eclipse Plus C₁₈ column (100 mm×3.0 mm, 1.8 μm) with mobile phases A (water containing 0.1% formic acid) and B (methanol) by gradient elution within 15 min, and detected under negative electrospray ionization (ESI) mode. The quantitative analysis was carried out by extracting the peak area with accurate mass. The confirmatory analysis of the target compounds was performed with the qualitative fragments. The results showed that the limits of quantification (LOQs, S/N=10) for the three target compounds were from 5.0 μg/kg to 10 μg/kg. The respective mean recoveries were found to be in the range of 79.1%-96.1%, and the RSDs were 5.7%-10.4%. It was applicable to the analysis of the three exogenous plant hormones in bean sprout samples. This method is simple, fast and efficient.
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