Citation: ZENG Yuzhu, ZHANG Yong, DONG Liangfei, ZHANG Beibei, ZHAO Yonggang. Simultaneous determination of six estrogens in different water bodies by solid phase extraction and ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(12): 1176-1181. doi: 10.3724/SP.J.1123.2013.08011 shu

Simultaneous determination of six estrogens in different water bodies by solid phase extraction and ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry

1.
1. School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China;

Corresponding author: ZHAO Yonggang,
Received Date: 13 August 2013
Available Online: 23 September 2013
Fund Project:

A method has been developed for the simultaneous determination of six estrogens (estriol, 17-β-estradiol, 17-α-estradiol, estrone, ethinyloestradiol, diethylstilbestrol) in water environment by solid phase extraction and ultra performance liquid chromatography couple with triple quadrupole mass spectrometry. After enriched and purified by an HLB solid phase extraction column, the six estrogens were separated by a BEH C18 column and then determined by MS/MS with multiple reaction monitoring (MRM) mode. The quantification was achieved by internal standard method, with estriol-D3, estradiol-D2 and diethylstilbestrol-D8 as the internal standards. Good linear relationships were obtained in the mass concentration range of 1.0-100 μg/L with high correlation coefficients over 0.9982. The limits of detection and quantification for the six estrogens were 0.27-0.45 ng/L and 1.08-1.78 ng/L, respectively. The recoveries were in the range of 68.3%-97.4% at the three spiked levels and with the relative standard deviations below 15%. The method shows high sensitivity, good precision, extensive analytical range and high analytical speed. It was successfully applied to the simultaneous detection of the six estrogens in four different water bodies including surface water, waste water, source water of drinking and domestic water.
- solid phase extraction (SPE),
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- estrogens,
- water bodies
1. [1]
  [1] Jobling S, Casey D, Rodgers-Gray T, et al. Aquat Toxicol, 2004, 66(2): 207
2. [2]
  [2] Yue Z F. Guide of Veterinary Drug Residues Detection in Food. Beijing: Standards Press of China (岳振峰. 食品中兽药残留检测指南. 北京: 中国标准出版社), 2010: 155
3. [3]
  [3] Duong C N, Ra J S, Cho J, et al. Chemosphere, 2010, 78(3): 286
4. [4]
  [4] Tan L C, Ge F, Shan Z J, et al. Journal of Ecology and Rural Environment (谭立超, 葛峰, 单正军, 等. 生态与农村环境学报), 2011, 27(5): 86
5. [5]
  [5] Lucci P, Nunez O, Galceran M T. J Chromatogr A, 2011, 1218(30): 4248
6. [6]
  [6] Liu R. Modern Preventive Medicine (刘韧. 现代预防医学), 2008, 35(23): 4567
7. [7]
  [7] Chang C C, Huang S D. Anal Chim Acta, 2010, 662(1): 39
8. [8]
  [8] Huang C, Jiang L Y, Chen J M, et al. Chinese Journal of Chromatography (黄成, 姜理英, 陈建孟, 等. 色谱), 2008, 26(5): 618
9. [9]
  [9] Zhang Y, Lan F, Zhang F, et al. Chinese Journal of Chromatography (张毅, 蓝芳, 张峰, 等. 色谱), 2011, 29(6): 523
10. [10]
  [10] Zhang H, Mao J, Sun C J, et al. Chinese Journal of Chromatography (张宏, 毛炯, 孙成均, 等. 色谱), 2003, 21(5): 451
11. [11]
  [11] Kozlik P, Bosakova Z, Tesarova E, et al. J Chromatogr A, 2011, 1218(15): 2127
12. [12]
  [12] Hong C Z, Yu X J, Yang W C, et al. J Chromatogr B, 2011, 879(28): 2998
13. [13]
  [13] Sun H W, Kang Z S, Li H. Chin J Anal Chem, 2010, 38(9): 1217
14. [14]
  [14] Baronti C, Curini R, Dascenzo G, et al. Environ Sci Technol, 2000, 34(24): 5059
15. [15]
  [15] Zhang S J, You J M, Ning S J, et al. J Chromatogr A, 2013, 1280: 84
16. [16]
  [16] Liu S, Ying G G, Zhao J L, et al. J Chromatogr A, 2011, 1218(10): 1367
17. [17]
  [17] Hu X X, Shi W, Cao F, et al. Chemosphere, 2013, 90(7): 2123
18. [18]
  [18] HJ 168-2010
19. [19]
  [19] Vallejo A, Fernandez L A, Olivares M, et al. J Chromatogr A, 2010, 1217 (52): 8327
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沈阳化工大学材料科学与工程学院沈阳 110142