Citation: LI Haoli, ZHAO Chunxia, ZHANG Junjie, FU Jiajun, WANG Ying, LU Xin, XU Guowang. Determination of twenty free amino acids in flue-cured tobacco leaves using ultra performance liquid chromatography-single quadruple mass spectrometry and pre-column derivatization[J]. Chinese Journal of Chromatography, ;2013, 31(12): 1182-1188. doi: 10.3724/SP.J.1123.2013.08006 shu

Determination of twenty free amino acids in flue-cured tobacco leaves using ultra performance liquid chromatography-single quadruple mass spectrometry and pre-column derivatization

LI Haoli ^1,2 ,
ZHAO Chunxia ¹ ,
ZHANG Junjie ¹ ,
FU Jiajun ² ,
WANG Ying ² ,
LU Xin ^1,, ,
XU Guowang ¹

1.
1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China;

Corresponding author: LU Xin,
Received Date: 2 August 2013
Available Online: 22 August 2013

Free amino acids in flue-cured tobacco leaves were investigated using the ultra performance liquid chromatography-single quadruple mass spectrometry detection and pre-column derivatization method. The validation results showed that the method could meet the analytical requirements. A total of 138 tobacco leaf samples were collected from 14 provinces in China in 2011 in which the free amino acids were determined. The relative standard deviations (RSDs) of the contents of free amino acids in different growing regions ranged from 28.50%-94.20%, and those of asparagine and glutamine were over 80%. The RSDs of the contents of free amino acids in full aroma tobacco leaves were larger than those in fresh aroma and medium aroma tobacco leaves. The principal component analysis (PCA) and non-parameter Mann-Whitney U test were used for data analysis. The free amino acids of the same aroma type grown in different regions or different aroma types in the same province showed great variation. The contents of free amino acids of full aroma tobacco grown in Southeast region were much lower than those in Huanghuai region. The contents of free amino acids in Hunan province were much lower than the average contents. The results showed that free amino acids in flue-cured tobacco leaves were affected by the growing region.
- ultra performance liquid chromatography (UPLC),
- single quadruple mass spectrometry,
- free amino acid,
- flue-cured tobacco,
- growing regions,
- aroma types
1. [1]
  [1] Liu R S. [MS Thesis]. Changsha: Hunan Agriculture University (刘荣森. [硕士学位论文]. 长沙: 湖南农业大学), 2007: 1
2. [2]
  [2] Liu R S, Zhang C S, Li J E, et al. Journal of Henan University of Science & Technology: Natural Science (刘荣森, 张长水, 李建娥, 等. 河南科技大学学报: 自然科学版), 2010, 31 (6): 66
3. [3]
  [3] Davis D L, Nielsen M T. Tobacco Production, Chemistry and Technology. Oxford, UK: Blackwell Science Ltd, 1999
4. [4]
  [4] Yu H, Mou S F. Chinese Journal of Analytical Chemistry (于泓, 牟世芬. 分析化学), 2005, 33(3): 398
5. [5]
  [5] Zhao T, Shi H Z, Ji X M, et al. Acta Tabacaria Sinica (赵田, 史宏志, 姬小明, 等. 中国烟草学报), 2011, 17(2): 13
6. [6]
  [6] Wang J, Zhang H, Guo G N, et al. Journal of Anhui Agricultural Sciences (王娟, 张华, 郭国宁, 等. 安徽农业科学), 2008, 36(26): 11413
7. [7]
  [7] Ali H, Patzold R, Brückner H. Food Chem, 2006, 99(4): 803
8. [8]
  [8] Kaspar H, Dettmer K, Gronwald W, et al. J Chromatogr B, 2008, 870(2): 222
9. [9]
  [9] Waldhier M C, Dettmer K, Gruber M A, et al. J Chromatogr B, 2010, 878(15/16): 1103
10. [10]
  [10] Li G, Wu D, Xie W Y, et al. J Chromatogr A, 2013, 1296: 243
11. [11]
  [11] Procopio S, Krause D, Hofmann T, et al. Food Sci Tech, 2013, 51(2): 423
12. [12]
  [12] Cho K, Kim Y, Wi S J, et al. J Agric Food Chem, 2012, 60: 11015
13. [13]
  [13] Huang Y F, Hu J. Chinese Journal of Chromatography (黄翼飞, 胡静. 色谱), 2010, 28(6): 615
14. [14]
  [14] Nimbalkar M S, Pai S R, Pawar N V, et al. Food Chem, 2012, 134(4): 2565
15. [15]
  [15] Han B, Wright R, Kirchhoff A M, et a1. Anal Biochem, 2013, 432(2): 74
16. [16]
  [16] Calderón-Santiago M, Priego-Capote F, Galache-Osuna J G, et al. J Pharm Biomed Anal, 2012, 70: 476
17. [17]
  [17] Zhang W Z, Kaye D M. Anal Biochem, 2004, 326(1): 87
18. [18]
  [18] Yu S X, Feng S, Sun Y S, et al. Chinese Journal of Chromatography (于淑新, 冯思, 孙元社, 等. 色谱), 2011, 29(3): 239
19. [19]
  [19] Sun L, Zhang X, Wang Y, et al. West China Journal of Pharmaceutical Sciences (孙莲, 张煊, 王岩, 等. 华西药学杂志), 2008, 23(4): 490
20. [20]
  [20] Li F L, Ji X M, Wei Y W, et al. Journal of Anhui Agricultural Sciences (李方楼, 姬小明, 魏跃伟, 等. 安徽农业科学), 2011, 39(27): 17010
21. [21]
  [21] Chen L M, Shang Y F, Zhao M B, et al. Chinese Journal of Chromatography (陈丽梅, 尚艳芬, 赵孟彬, 等. 色谱), 2010, 28(12): 1154
22. [22]
  [22] Bosch L, Alegría A, Farré R. J Chromatogr B, 2006, 831(1/2): 176
23. [23]
  [23] Yang D L, Wang S S, Wang B H, et al. Chinese Tobacco Science (杨德廉, 王树声, 王宝华, 等. 中国烟草科学), 1998(3): 11
24. [24]
  [24] Gong C R, Liu D Y. Chinese Tobacco Science (宫长荣, 刘东洋. 中国烟草科学), 2003, 24 (1): 1
25. [25]
  [25] Zhang J J, Zhao C X, Chang Y W, et al. J Sep Sci, 2013, 36(17): 2868
26. [26]
  [26] Ding Y F, Li Y P, Zhang X Q, et al. Acta Agriculturae Boreali-occidentalis Sinica (丁燕芳, 李亚培, 张小全, 等. 西北农业学报), 2012, 21(3): 97
27. [27]
  [27] Zhu Z Q. Acta Tabacaria Sinica (朱尊权. 中国烟草学报), 2008, 14(6): 70
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