Citation: CHEN Dawei, MIAO Hong, ZHAO Yunfeng, WU Yongning. Effect of alcoholic strength on the determination of ethyl carbamate in Chinese spirits by high performance liquid chromatography-fluorescence detection[J]. Chinese Journal of Chromatography, ;2013, 31(12): 1206-1210. doi: 10.3724/SP.J.1123.2013.07023 shu

Effect of alcoholic strength on the determination of ethyl carbamate in Chinese spirits by high performance liquid chromatography-fluorescence detection

1.
China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China

Corresponding author: ZHAO Yunfeng,
Received Date: 17 July 2013
Available Online: 31 July 2013
Fund Project:

A high performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for rapid determination of ethyl carbamate (EC) in Chinese spirits. Through the analysis and comparison of the EC peak areas in different alcoholic strengths determined by HPLC-FLD, the effect of alcoholic strength on the determination of the content of EC was found. The alcoholic strength and the peak area of EC showed good linearity in the range of 5% to 65% (v/v) alcohol content, and the correlation coefficients (R²) were higher than 0.98. Furthermore, the conversion between the peak area of EC with different alcoholic strengths was established by the relative correction coefficient. The method showed a good linearity in the range of 10 to 500 μg/L for EC with the average recoveries of 98.9%-108.2% and RSDs of 0.6%-4.9%. The reliability of the established HPLC-FLD method was evaluated by comparison with GC-MS method. The results showed that the results of two methods were not significantly different. The developed method is simple, sensitive, accurate, and suitable for the rapid determination of EC in Chinese spirits.
- high performance liquid chromatography (HPLC),
- ethyl carbamate (EC),
- alcoholic strength,
- Chinese spirits
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沈阳化工大学材料科学与工程学院沈阳 110142