Citation: LIU Haishan, QIAN Xiaoyan, LÜ Chunhua, ZHU Xiaoyu, CHEN Xiaomei, MO Weimin. Simultaneous determination of 10 synthetic colorants in cosmetics by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(11): 1106-1111. doi: 10.3724/SP.J.1123.2013.05018 shu

Simultaneous determination of 10 synthetic colorants in cosmetics by high performance liquid chromatography

LIU Haishan ^1,3,, ,
QIAN Xiaoyan ² ,
LÜ Chunhua ^1,3 ,
ZHU Xiaoyu ^1,3 ,
CHEN Xiaomei ¹ ,
MO Weimin ²

1.
1. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, China;

Corresponding author: LIU Haishan,
Received Date: 9 May 2013
Available Online: 2 July 2013
Fund Project: 浙江出入境检验检疫局课题项目(2012B119). (2012B119)

A high performance liquid chromatographic method was developed for the simultaneous determination of 10 synthetic colorants in cosmetics. The cosmetics were extracted by the ultrasonic technique with tetrahydrofuran (THF), dimethyl sulfoxide (DMSO) and methanol sequentially. Then the extracts were centrifuged for purification and separated on an Eclipse XDB-C18 column (150 mm×4.6 mm, 5 μm) with gradient elution by acetonitrile and 0.02 mol/L ammonium acetate (pH 4.60, adjusted with acetic acid). A diode array detector was used to determine the colorants with the wavelengths ranging from 417 nm to 640 nm. The linear relationships of the 10 colorants between the peak areas and the mass concentrations were obtained in the range of 0.5-20.0 mg/L (r >0.999). The limits of quantitation ranged from 10 to 20 mg/kg. The average recoveries at three concentration levels ranged from 92.9% to 108.8% with the relative standard deviations in the range of 0.5% to 6.1% (n=6). The method is simple, rapid and sensitive. It is suitable for the simultaneous determination of the 10 colorants in the oil cosmetics, cream cosmetics and powder cosmetics.
- high performance liquid chromatography (HPLC),
- colorants,
- cosmetics
1. [1]
  [1] Tomankova K, Kejlova K, Binder S. Toxicol In Vitro, 2011, 25: 1242
2. [2]
  [2] Chen R Q. Dyeing and Finishing (陈荣圻. 印染), 2009(21): 46
3. [3]
  [3] Ministry of Health of the People's Republic of China. Hygienic Standard for Cosmetics (中华人民共和国卫生部. 化妆品卫生规范). (2007-01-04) [2012-02-10]. http://www.moh.gov.cn/open/web_edit_file/20070124145740.pdf
4. [4]
  [4] European Commission. Consolidated Version of 76/768/EEC: Council Directive of 27 July 1976 on the Approximation of the Laws of the Member States Relating to Cosmetic Products. (2012-08-23) [2013-04-07]. http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CONSLEG: 1976L0768: 20120823:EN:PDF
5. [5]
  [5] U. S. Food and Drug Administration. Color Additives Permitted for Use in Cosmetics. [2013-04-07]. http://www.fda.gov/Cosmetics/GuidanceComplianceRegulatoryInformation/VoluntaryCosmeticsRegistrationProgramVCRP/OnlineRegistration/ucm109084.htm
6. [6]
  [6] Ge B K, Chen Q Y, Zhao K X. Journal of Environment and Health (葛宝坤, 陈其勇, 赵孔祥. 环境与健康杂志), 2007, 24(11): 917
7. [7]
  [7] Rastogi S C, Barvick V J, Carter S V. Chromatographia, 1997, 45(1): 215
8. [8]
  [8] Wu K S, Wu A B, Huang M C, et al. Journal of Food and Drug Analysis (吴坤善, 吴安邦, 黄明权, 等. 药物食品分析), 1999, 7(2): 95
9. [9]
  [9] Li Y, Sun X Y, Zhang C, et al. Journal of Instrumental Analysis (李英, 孙小颖, 张琛, 等. 分析测试学报), 2009, 28(8): 958
10. [10]
  [10] Zhu J M, Xie J F, Du D A. Preventive Medicine Tribune (朱杰民, 谢剑锋, 杜达安. 预防医学论坛), 2005, 11(4): 423
11. [11]
  [11] Ma Y F, Li Q, Wu X J. China Surfactant Detergent & Cosmetics (马艳凤, 李琼, 武晓剑. 日用化学工业), 2011, 41(6): 455
12. [12]
  [12] Lu J, Li M H. Flavour Fragrance Cosmetics (卢剑, 郦明浩. 香料香精化妆品), 2008(1): 20
13. [13]
  [13] Lin W X, Sun X Q, Zhao X R, et al. Chinese Journal of Chromatography (林维宣, 孙兴权, 赵雪蓉, 等. 色谱), 2012, 30(5): 527
14. [14]
  [14] Soylak M, Unsal Y E, Yilmaz E, et al. Food Chem Toxicol, 2011, 49: 1796
15. [15]
  [15] Wang C C, Masi A N, Fernández L. Talanta, 2008, 75: 135
16. [16]
  [16] Chen L J, Huang J F, He M H, et al. Chinese Journal of Chromatography (陈立坚, 黄金凤, 何敏恒, 等. 色谱), 2012, 30(6): 630
17. [17]
  [17] Noguerol-Cal R, López-Vilariño J M, Fernández-Martínez G, et al. J Chromatogr A, 2008, 1179: 152
18. [18]
  [18] Bai H, Chen W, Liu J. Chinese Journal of Analysis Laboratory (白桦, 陈伟, 刘娟. 分析试验室), 2006, 25(4): 93
19. [19]
  [19] Wang Y, Wu N. Journal of Anhui Agricultural Sciences (王勇, 武娜. 安徽农业科学), 2012, 40(15): 8723
20. [20]
  [20] Tan J L, Su Z B, Han Y, et al. Journal of Anhui Agricultural Sciences (谭建林, 苏钟璧, 韩熠, 等. 安徽农业科学), 2012, 40(16): 8954
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