Citation: WANG Yu-Hong, LIU Fang, ZHAO Hui, WANG Jian-Min, WANG Yan, YAN Chao. Development and Evaluation of Micro Evaporative Light Scattering Detector[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1622-1626. doi: 10.3724/SP.J.1096.2012.20373 shu

Development and Evaluation of Micro Evaporative Light Scattering Detector

WANG Yu-Hong ^1,2,3 ,
LIU Fang ² ,
ZHAO Hui ² ,
WANG Jian-Min ³ ,
WANG Yan ² ,
YAN Chao ^1,2,3,,

1.
¹ Department of Chemistry, East China Normal University, Shanghai 200062, China;

Corresponding author: YAN Chao,
Received Date: 9 April 2012
Available Online: 20 May 2012
Fund Project: 本文系国家重大科学仪器设备开发专项项目(No.2011YQ150072) (No.2011YQ150072) 国家自然科学基金项目(No.21175092) (No.21175092)上海市引进技术的吸收与创新计划项目(No.JJ-YJCX-03-10-2586)资助 (No.JJ-YJCX-03-10-2586)

A novel micro-flow evaporative light scattering detector (μELSD), suitable for capillary liquid chromatography (cLC) system, was constructed and eveluated. The main parameters of detector, such as the aperture of nebulizer, inner diameter of capillary, specification of the evaporative tube and scattering cell, were optimized. Under the optimal experimental conditions:nebulizer 410 μm I.D., nebulized capillary 50 μm I.D.×360 μm O.D., evaporative tube 15 cm×4.6 mm I.D., scattering cell 60 mm×33 mm I.D., the detection limitation of glucose (S/N＞10) was 1 ng with a linear range of 0.01-1 μg. Good reproducibility has been achieved in peak area and peak height with the RSD (n=6) of 0.4% and 0.3% (n=6), respectively. The detector was successfully coupled to a cLC system and applied to the separation and detection of three kinds of sweeteners, in the condition of capillary column ( C₁₈, 250 μm I.D.) and 0.1% (V/V) ammonium formate (pH=4.5): methanol (60:40, V/V) as mobile phase. The cLC-μELSD system has the advantages in universal applicability, high column efficiency, and less solvent and sample consumptions compare with a common HPLC-ELSD system, and is suitable for separation and analysis of real samples.
- Evaporative light scattering detector,
- Capillary liquid chromatography,
- Miniaturization,
- Sweeteners
1. [1]
  1 Chariesworth J M. Anal. Chem., 1978, 50: 1414-1420
2. [2]
  2 Stolyhwo A, Colin H, Guiochon G. J. Chromatogr., 1983, 265: 1-18
3. [3]
  3 Stolyhwo A, Colin H, Martin M, Guiochon G. J. Chromatogr., 1984, 288: 253-275
4. [4]
  4 Stolyhwo A, Colin H, Guiochon G. Anal. Chem., 1985, 57: 1342-1354
5. [5]
  5 ZHAO Shao-Hua, LIU Min-Yan, WANG Yu-Feng, XU Hong-Hhui, ZHANG Yong-Feng, WANG Meng. Food Science, 2012, 33(02): 224-226
6. [6]
  赵韶华, 刘敏彦, 王玉峰, 许红辉, 张永锋, 王猛. 食品科学, 2012, 33(2): 224-226
7. [7]
  6 LIU Fang, WANG Yan, WANG Yu-Hong, ZHOU Jun-Yi, YAN Chao. Chinese Journal of Chromatography, 2012, 30(3): 292-297
8. [8]
  刘芳, 王彦, 王玉红, 周君裔, 阎超. 色谱, 2012, 30(3): 292-297
9. [9]
  7 LIU Wei, JIN Cheng, KONG Wei-Jun, WANG Hai-Tao, GONG Qian-Feng, XIAO Xiao-He. Chinese J. Anal. Chem., 2010, 38(4):522-526
10. [10]
  刘苇, 金城, 孔维军, 王海涛, 龚千锋, 肖小河. 分析化学, 2010, 38(4): 522-526
11. [11]
  8 WANG Yu-Hong, SHEN Ke-Yu, LI Peng, ZHOU Jun-Yi, YAN Chao. Chinese Journal of Chromatography, 2011, 29(9): 908-911
12. [12]
  王玉红, 申克宇, 李鹏, 周君裔, 阎超. 色谱, 2011, 29(9): 908-911
13. [13]
  9 ZHANG Yong, ZHOU Ya-Qiu, CHEN Fa-Gui.Chinese Journal of Pharmaceutical Analysis, 2008, 28(10): 1732-1734
14. [14]
  张勇, 周亚球, 陈法贵. 药物分析杂志, 2008, 28(10): 1732-1734
15. [15]
  10 James N A. J. Microcolumn Separations, 1998, 10(6): 491-502
16. [16]
  11 Magnus B O A, Lars G B. J. Microcolumn Separations, 1998, 10(3): 249-254
17. [17]
  12 Karen G, Arlette B, Pierre C. J. Chromatogr. A, 2004, 1051(1-2): 43-51
18. [18]
  13 Loic Q, Karen G, Arlette B, Pierre C. J. Separation Science, 2006, 29(3): 390-398
19. [19]
  14 Guillarme D, Rudaz S, Schelling C, Dreux M, Veuthey J L. J. Chromatogr. A, 2008, 1192(1): 103-112
1. [1]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
2. [2]
  Jiandong Liu , Xin Li , Daxiong Wu , Huaping Wang , Junda Huang , Jianmin Ma . Anion-Acceptor Electrolyte Additive Strategy for Optimizing Electrolyte Solvation Characteristics and Electrode Electrolyte Interphases for Li||NCM811 Battery. Acta Physico-Chimica Sinica, 2024, 40(6): 2306039-0. doi: 10.3866/PKU.WHXB202306039
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
5. [5]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
6. [6]
  Hongxia Yan , Rui Wu , Weixu Feng , Yan Zhao , Yi Yan . Innovation Inspired by Classical Chemistry: Luminescent Hyperbranched Polysiloxanes. University Chemistry, 2025, 40(4): 154-159. doi: 10.12461/PKU.DXHX202409010
7. [7]
  Yingpeng ZHANG , Xingxing LI , Yunshang YANG , Zhidong TENG . A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1301-1308. doi: 10.11862/CJIC.20250064
8. [8]
  Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
9. [9]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
10. [10]
  Zhuo Han , Danfeng Zhang , Haixian Wang , Guorui Zheng , Ming Liu , Yanbing He . Research Progress and Prospect on Electrolyte Additives for Interface Reconstruction of Long-Life Ni-Rich Lithium Batteries. Acta Physico-Chimica Sinica, 2024, 40(9): 2307034-0. doi: 10.3866/PKU.WHXB202307034
11. [11]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
12. [12]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
13. [13]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
14. [14]
  Kangjuan Cheng , Chunxiao Liu , Youpeng Wang , Qiu Jiang , Tingting Zheng , Xu Li , Chuan Xia . Design of noble metal catalysts and reactors for the electrosynthesis of hydrogen peroxide. Acta Physico-Chimica Sinica, 2025, 41(10): 100112-0. doi: 10.1016/j.actphy.2025.100112
15. [15]
  Qianli Ma , Tianbing Song , Tianle He , Xirong Zhang , Huanming Xiong . Sulfur-doped carbon dots: a novel bifunctional electrolyte additive for high-performance aqueous zinc-ion batteries. Acta Physico-Chimica Sinica, 2025, 41(9): 100106-0. doi: 10.1016/j.actphy.2025.100106
16. [16]
  Aoyu Huang , Jun Xu , Yu Huang , Gui Chu , Mao Wang , Lili Wang , Yongqi Sun , Zhen Jiang , Xiaobo Zhu . Tailoring Electrode-Electrolyte Interfaces via a Simple Slurry Additive for Stable High-Voltage Lithium-Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(4): 100037-0. doi: 10.3866/PKU.WHXB202408007
17. [17]
  Yi Fan , Zhuoqi Jiang , Zhipeng Li , Xuan Zhou , Jingan Lin , Laiying Zhang , Xu Hou . 偶极诱导液体门控可视化物质检测——化学“101计划”表界面性质应用实验新设计. University Chemistry, 2025, 40(8): 265-271. doi: 10.12461/PKU.DXHX202410061
18. [18]
  Jia-He Li , Yu-Ze Liu , Jia-Hui Ma , Qing-Xiao Tong , Jian-Ji Zhong , Jing-Xin Jian . 洛芬碱衍生物的合成、化学发光与重金属离子检测. University Chemistry, 2025, 40(6): 230-237. doi: 10.12461/PKU.DXHX202407080
19. [19]
  Cun WANG , Shaohan XU , Yuqian ZHANG , Yaoyao ZHANG , Tao GONG , Rong WEN , Yuhang LIAO , Yanrong REN . Terbium complex electrochemiluminescent emitters: Synthesis and application in the detection of epinephrine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1351-1360. doi: 10.11862/CJIC.20240427
20. [20]
  Pengli GUAN , Renhu BAI , Xiuling SUN , Bin LIU . Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1817-1826. doi: 10.11862/CJIC.20250058

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(564)
HTML views(18)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142