Citation: LV Cui, KANG Tian-Fang, LU Li-Ping, XIONG Yue. Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1822-1826. doi: 10.3724/SP.J.1096.2012.20303 shu

Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction

1.
College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Corresponding author: KANG Tian-Fang,
Received Date: 23 March 2012
Available Online: 6 July 2012
Fund Project: 本文系北京市教委科技发展基金重点项目(No.KZ201110005006) (No.KZ201110005006)北京市自然科学基金(No.8102009) (No.8102009)北京市人才强教深化计划——创新团队(No.PHR201007105)资助 (No.PHR201007105)

Layer-by-layer films of poly dimethyl diallyl ammonium chloride and DNA were assembled on the surface of a glassy carbon electrode to prepare DNA electrochemical sensor. The assembling of DNA was characterized by employing electrochemical methods and atomic force microscope. We used Ru(bpy)₃²⁺ as electrochemical catalyst to detect the oxidative damage of DNA in the membrane. The effects of incubation time and concentration of Fenton reagent on the damage of DNA were studied. The result showed that DNA could be damaged greatly when it was incubated in Fenton reagent for 15 min. The detectable concentration of Fenton reagent was 5.0×10^-5 mol/L FeSO₄/2.0×10^-4 mol/L H₂O₂. The electrochemical method using Ru(bpy)₃²⁺ as catalyst possesses high-sensitivity for detecting the damage of DNA.
- Fenton reaction,
- Deoxyribonucleic acid damage assembly,
- Layer-by-layer assembly,
- Deoxyribonucleic acid electrochemical sensors
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沈阳化工大学材料科学与工程学院沈阳 110142