Citation: XU Yang, ZOU Long, LIU Shi-Wen, LIU Jing, CHEN Bo. Production of Monoclonal Antibodies for Detection of Fumonisin B1 by Chemiluminescence Enzyme-linked Immunosorbent Assaay[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(11): 1735-1739. doi: 10.3724/SP.J.1096.2012.20201 shu

Production of Monoclonal Antibodies for Detection of Fumonisin B1 by Chemiluminescence Enzyme-linked Immunosorbent Assaay

XU Yang ^1,, ,
ZOU Long ¹ ,
LIU Shi-Wen ² ,
LIU Jing ¹ ,
CHEN Bo ¹

1.
¹ State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China;

Corresponding author: XU Yang,
Received Date: 29 February 2012
Available Online: 4 June 2012
Fund Project: 本文系国家"863"计划资助项目(No. 2007AA10Z427) (No. 2007AA10Z427)

Based on monoclonal antibodies with high affinity against fumonisin B1, a direct competitive chemiluminescence enzyme-linked immunosorbent assay (dc-CLEIA) for detection of FB₁ in cereal was developed. FB₁ artificial antigens were synthesized by the carbodiimide crosslinking methods, and then a hybridoma cell that secreted the antibody selectively identifies FB₁ was selected through cell fusion. The monoclonal antibody titer was found as higher as 1.28×10⁶, which was labeled with horse radish peroxidase (HRP) through sodium periodate activation for development of dc-CELIA. A good linearity was achieved over a concentration range of 0.32-8.40 μg/L, the IC50 value and the limit of detection were 1.43 μg/L and 0.13 μg/L, respectively. Meanwhile, the competitive reaction time was only 20 min. The recoveries of FB₁ in all tissues were between 100.2%-115.4% and the relative standard deviation was less than 8.7%. The dc-CLEIA was applied in quantification of FB₁ elimination in cereal, the results showed good correlations of the results obtained by HPLC and a commercial ELISA kit.
- Fumonisin B1,
- Monoclonal antibody,
- Chemiluminescence enzyme immuno sorbant assay,
- Cereal
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沈阳化工大学材料科学与工程学院沈阳 110142