Citation: LI Xue, GAO Guo-Ming, NIU Li-Yuan, LIN Man-Man, QIN Zong-Ding, LIU Jun-Xian, YAO Hui-Lu. In Vivo Raman Imaging of Mice Ear[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1494-1499. doi: 10.3724/SP.J.1096.2012.20168 shu

In Vivo Raman Imaging of Mice Ear

LI Xue ^1,2 ,
GAO Guo-Ming ³ ,
NIU Li-Yuan ^1,2 ,
LIN Man-Man ^1,2 ,
QIN Zong-Ding ^1,2 ,
LIU Jun-Xian ¹ ,
YAO Hui-Lu ^2,,

1.
¹ College of Physics & Technology, Guangxi Normal University, Guilin 541004, China;

Corresponding author: YAO Hui-Lu,
Received Date: 23 February 2012
Available Online: 12 April 2012
Fund Project: 本文系国家自然科学基金(Nos.60868002,31060128) (Nos.60868002,31060128)广西自然科学基金(Nos.2012GXNSFFA060008,2011GXNSFA018143,0731013)资助 (Nos.2012GXNSFFA060008,2011GXNSFA018143,0731013)

Raman spectra had been used to image the spatial distribution of chemical composition by scanning the laser spot across some parts of mice ear at different depth. Selecting the bands at 1125 cm^-1(blood sugar), 1300 cm^-1(lipids), 1549 cm^-1 (hemoglobin), 1660 cm^-1 (protein) to calculate their peak areas, and using these data to generate 2D, and 3D images, we have demonstrated that Raman imaging can yield 3D spatially biochemical information from a living tissue. This technique provides a new way to study the biochemical molecule's distribution in vivo.
- Spectroscopy,
- Raman spectrum,
- Imaging,
- In vivo
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