Citation: YU Hui-Juan, LI Bing, CAI You-Qiong, YE Fang-Ting, TAI Jian-Ming, HUI Yun-Hua, XU Jie, FENG Bing. Determination of Semicarbazide Content in Crustaceans by Liquid Chromatography Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1530-1535. doi: 10.3724/SP.J.1096.2012.20160 shu

Determination of Semicarbazide Content in Crustaceans by Liquid Chromatography Tandem Mass Spectrometry

YU Hui-Juan ^1,, ,
LI Bing ² ,
CAI You-Qiong ¹ ,
YE Fang-Ting ³ ,
TAI Jian-Ming ⁴ ,
HUI Yun-Hua ¹ ,
XU Jie ¹ ,
FENG Bing ¹

1.
¹ Fishery Products Quality Inspection and Test Center (Shanghai) of Ministry of Agiculture, Shanghai 200090, China;

Corresponding author: YU Hui-Juan,
Received Date: 22 February 2012
Available Online: 25 May 2012
Fund Project: 本文系农业部财政专项(No.2130109)资助 (No.2130109)

The content of semicarbazide in crustaceans had been determined by LC/MS/MS. Samples were hydrolyzed by hydrochloric acid and derivatized with 2-nitrobenzaidehyde. After extraction with ethyl acetate, chromatographic separation was achieved by HPLC and the target compound was detected by tandem mass spectrometry using internal standard as quantification method. The qualitative limit was 0.25 μg/kg(S/N＞3)and the quantitative limit was 0.5 μg/kg(S/N＞10). Crustacean species were from natural water and free market of agricultural products including oriental river prawn, giant tiger prawn, giant freshwater prawn, white-leg shrimp, red swamp crayfish, mud crab, dungeness crab, swimming crab, mitten crab and horse shoe crab. Concentrations of semicarbazide in shrimp and in crab were from not detected to 370.4 μg/kg and not detected to 87.5 μg/kg and concentrations of semicarbazide varied from tissues with highest in carapace and lowest in muscle. In the present study, the results revealed that semicarbazide was an endogenous substance existing in crustaceans.
- Semicarbazide,
- Endogenous substance,
- Crustacean aquatic products,
- Distribution characteristics
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