Citation: ZHOU Lin-Ting, LI Zai-Jun, FANG Yin-Jun. Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B₁[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(11): 1635-1641. doi: 10.3724/SP.J.1096.2012.20151 shu

Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B₁

1.
¹ School of Chemical and Materials Engineering, Jiangnan University, Wuxi 214122, China;

Corresponding author: LI Zai-Jun,
Received Date: 21 February 2012
Available Online: 21 May 2012
Fund Project: 本文系国家科技支撑计划(No.2011BAK10B032) (No.2011BAK10B032)国家自然科学基金(No.21176101) (No.21176101)浙江省自然科学基金(No.Y4100729) (No.Y4100729)

To fabricate the aflatoxin B₁ immunosensor, graphene oxide, 2,5-di-(2-thienyl)-1-pyrrole-1-(p-benzoic acid) and chloroauric acid were electrodeposited on the gold electrode surface in sequence. Then aflatoxin B₁ (AFB₁) antibody was covalently connected to the conducting polymer film with 1-ethyl-(3-dimethyl-aminopropyl)-carbodimide/N-hydroxysuccimimide (EHC/NHS) as activator and 1,3-dibutyliminazolium hexafluorophosphate ionic liquid was finally coated on the modified electrode. The Fe(CN)₆^3-/4- phosphate buffer solution (pH 7.0) was employed as base solution for investigating electrochemical performances of the immunosensor by cyclic voltammetry and electrochemical impedance spectroscopy. Research revealed the introduction of graphene and gold nanocomposite obviously improved electron transfer rate of the modified layer, and the apparent electroactive surface areas of the electrode also increased up to 0.2188 cm² and 0.2640 cm² from 0.1772 cm² of bare gold electrode, respectively. When aflatoxin B₁ concentration is in the range of 3.2×10^-15-3.2×10^-13 mol/L, the electron transfer impedance responses of the sensor will linearly increase. The correlation coefficient (R²) and the detection limit were found to be 0.994 and 1.1×10^-15 mol/L. The electrochemical response of the immunosenor can keep almost constant after stored at 4℃ for 20 weeks. Sensitivity and stability of the proposed method are better than those of other methods reported in literatures, it has been successfully applied to determination of trace AFB₁ in peanut samples.
- Conducting polymer,
- Graphene,
- Gold nanoparticles,
- Electrodeposition,
- Immunosensor,
- Aflatoxin B₁
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沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B1

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Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B₁