Citation: ZHAO Yan-Hua, LIN Ni-Ni, GUO Lei, CHEN Jia, LIU Wan-Hui, XIE Jian-Wei. Simultaneous Determination of T-2 Toxin and Its Metabolites in Rat Plasma Using Solid Phase Extraction and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1852-1858. doi: 10.3724/SP.J.1096.2012.20124 shu

Simultaneous Determination of T-2 Toxin and Its Metabolites in Rat Plasma Using Solid Phase Extraction and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHAO Yan-Hua ^1,2 ,
LIN Ni-Ni ¹ ,
GUO Lei ¹ ,
CHEN Jia ¹ ,
LIU Wan-Hui ² ,
XIE Jian-Wei ^1,,

1.
¹ College of Pharmacy, Yantai University, Yantai 264005, China;

Corresponding author: XIE Jian-Wei,
Received Date: 15 March 2012
Available Online: 22 May 2012
Fund Project: 本文系"十二五"国家科技支撑计划(No.2011BAK10B07)资助项目 (No.2011BAK10B07)

T-2 toxin and its 5 metabolites in rat plasma were simultaneously determined by an ultra performance liquid chromatography coupled with triple quadrupole-ion trap mass spectrometric (UPLC-QTRAP) method with high sensitivity. In the optimized solid phase extraction conditions, the rat plasma samples were purified and concentrated by a HLB column, and then the analytes were separated on a XDB-C18 column (4.6 mm×50 mm, 1.8 μm) with a mobile phase of methanol and aqueous solution containing 5 mmol/L ammonium acetate under a gradient elution. The mass spectrometric identification and quantification were performed with electrospray ionization in a positive by using multiple reaction monitoring mode with zearalanone as internal standard. In this established method, good linearities (R²>0.997) in the range from 0.1 to 500 μg/L with the limits of detection between 0.02 to 0.3 μg/L, and the recoveries varied from 93.5% to 120.3% with a relative standard deviations less than 13% were obtained. The method is rapid, reproducible, sensitive, and can be applied in the retrospective determination of the intoxication event of T-2 toxin.
- T-2 toxin,
- Metabolites,
- Solid phase extraction,
- Ultra performance liquid chromatography tandem mass spectrometry,
- Plasma
1. [1]
  1 WANG Min-Hui, LI Lü-Mu, DING Xiao-Ling. Chinese Journal of Animal Nutrition., 2011, 23(1): 20-24
2. [2]
  王敏辉, 李吕木, 丁小玲. 动物营养学报, 2011, 23(1): 20-24
3. [3]
  2 Li Y S, Wang Z H, Beier R C, Shen J Z, de Smet D, de Saeger S, Zhang S X. J. Agric. Food Chem., 2011, 59(8): 3441-3453
4. [4]
  3 Tolerance Limits for T-2 Toxin in Formula Feed. The People's Republic of China National Standard. GB 21693- 2008
5. [5]
  配合饲料中T-2毒素的允许量. 中华人民共和国国家标准, GB 21693- 2008
6. [6]
  4 Wu Q H, Dohnal V, Huang L L, Kuúa K, Yuan Z H. Drug Metabolism Reviews, 2010, 42(2): 250-267
7. [7]
  5 PENG Shuang-Qing, DONG Jian-Ying, YANG Jin-Sheng. Chinese Journal of Preventive Medicine., 1996, 30(3): 141-143
8. [8]
  彭双清, 董剑英, 杨进生. 中国预防医学杂志, 1996, 30(3): 141-143
9. [9]
  6 Yoshizawa T, Onomoto C, Morooka N. Appl. Environ. Microbiol., 1980, 39(5): 962-966
10. [10]
  7 Sibanda L, de Saeger S, van Peteghem C, Grabarkiewicz-Szczesna J, Tomczak M. J. Agric. Food Chem., 2000, 48(2): 5864-5867
11. [11]
  8 Kotal F, Holadová K, Hajlová J, Poustka J, Radova Z. J. Chromatogr. A, 1999, 830(1): 219-225
12. [12]
  9 Schollenberger M, Lauber U, Terry Jara H, Drochner W, Müller H M. J. Chromatogr. A, 1998, 815(1): 123-132
13. [13]
  10 Ibáñez-Vea M, Lizarraga E, Gonzalez-Peñas E. Food Control., 2011, 22(8): 1428-1434
14. [14]
  11 Nielsen K F, Thrane U. J. Chromatogr. A, 2001, 929(1-2): 75-87
15. [15]
  12 Medina A, Valle-Algarra F M, Jimnez M, Magan N. J. Chromatogr. B, 2010, 878: 2145-2149
16. [16]
  13 Visconti A, Lattanzio, V M T, Pascale M, Haidukowskie M. J. Chromatogr. A, 2005, 1075(1-2): 151-158
17. [17]
  14 Trebstein A, Seefelder W, Lauber U, Humpf H-U. J. Agric. Food Chem., 2008, 56(13): 4968-4975
18. [18]
  15 Dall'Asta C, Sforza S, Galaverna G, Dossena A, Marchelli R. J. Chromatogr. A, 2004, 1054(1-2): 389-395
19. [19]
  16 Klötzel M, Gutsche B, Lauber U, Humpf H-U. J. Agric. Food Chem., 2005, 53(23): 8904-8910
20. [20]
  17 Berthiller F, Schuhmacher R, Buttinger G, Krska R. J. Chromatogr. A, 2005, 1062(2): 209-216
21. [21]
  18 Schuhmacher R, Berthiller F, Buttinger G, Krska R. Mycotoxin Research, 2005, 21(4): 237-240
22. [22]
  19 Delmulle B, de Saeger S, Adams A, de Kimpe N, van Peteghem C. Rapid Commun. Mass Spectrom., 2006, 20(5): 771-776
23. [23]
  20 Diana Di Mavungu J, Monbaliu S, Scippo M-L, Maghuin-Rogister G, Schneider Y-G , Larondelle Y, Callebaut A , Robbens J, Van Peteghem C, De Saeger S. Food Additives & Contaminants, 2009, 26(6): 885-895
24. [24]
  21 YING Yong-Fei, ZHU Cong-Ying, WEI Min-Jue, CHEN Hui-Hua. Chinese J. Anal. Chem., 2010, 38(12): 1759-1764
25. [25]
  应永飞, 朱聪英, 韦敏珏, 陈慧华. 分析化学, 2010, 38(12): 1759-1764
26. [26]
  22 Aguilera-Luiz M M, Plaza-Bolaos P, Romero-González R, Martínez Vidal J L, Garrido Frenich A. Anal. Bioanal Chem., 2011, 399: 2863-2875
27. [27]
  23 Rubert J, Soriano J M, Maes J, Soler C. Food Chem. Toxicol., 2011, 49(9): 2299-2304
28. [28]
  24 De Baere S, Goossens J, Osselaere A, Devreese M, Vandenbroucke V, de Backer P, Croubels S. J. Chromatogr. B, 2011, 879(24): 2403-2415
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