Citation: SHI Jing-Jing, LIAN Jie, ZHOU Wen-Wen, SHI Xi-Zeng, GAO Yun-Hua. Magnetoresistive Biosensor for Detection of Chloramphenicol Residue in Milk[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1524-1529. doi: 10.3724/SP.J.1096.2012.20046 shu

Magnetoresistive Biosensor for Detection of Chloramphenicol Residue in Milk

1.
¹ Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China;

Corresponding author: GAO Yun-Hua,
Received Date: 13 January 2012
Available Online: 18 April 2012
Fund Project: 本文系国家自然科学基金(No.20975106) (No.20975106) 中国科学院知识创新工程重要方向项目(No.KJCX2-YW-M15) (No.KJCX2-YW-M15)广东省中国科学院全面战略合作项目(No.2011A090100036)资助 (No.2011A090100036)

Based on direct competitive immunoassay principle and giant magnetoresistance (GMR) effect, a novel method for detecting chloramphenicol (CAP) by magnetic biosensor has been developed. The sample, biotinylated anti-CAP monoclonal antibody, and streptavidin-beads conjugate were added onto the prepared CAP hapten chips one by one. Subsequently, competitive immune reaction happened and the number of beads bonded on chips was detected by magnetic biosensor. The standard curve was established by optimizing the detection condition. This method can be used to detect CAP in a range of 0.05-100.0 μg/L, detection limit of 50 ng/L. The analyte recoveries in milk samples ranged from 95.97% to 99.36%. The relative standard deviations ranged from 0.8% to 3.9% for intra-assay and 1.1% to 1.7% for inter-assay. The correlation coefficient between this method and ELISA was 0.98. Rapid quantitative detection could be accomplished within 30 minutes using this method. It provides feasibility for the establishment of rapid multi-target magnetic competitive immunoassay system.
- Magnetic competitive immunoassay,
- Magnetic biosensor,
- Chloramphenicol,
- Number of beads
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沈阳化工大学材料科学与工程学院沈阳 110142