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Citation: FU Shenghui, LIU Jian, LIU Shaopu. Fluorescence Quenching Reaction of Ceftriaxone with Acriflavine and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, ;2014, 31(7): 865-870. doi: 10.3724/SP.J.1095.2014.30472 shu

Fluorescence Quenching Reaction of Ceftriaxone with Acriflavine and Its Analytical Application

FU Shenghui ^a,b ,
LIU Jian ^c ,
LIU Shaopu ^a,,

1.
a School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715, China;
2.
b Chongqing Ba'nan District Environmental Monitor Station, Chongqing 401320, China;
3.
c Chongqing Jiulongpo District Environmental Monitor Station, Chongqing 400050, China

Corresponding author: LIU Shaopu,
Received Date: 22 September 2013
Available Online: 17 October 2013
Fund Project: 国家自然科学基金资助项目(20875078) (20875078)

In pH 2.09~5.02 Britton-Robinson(BR) buffer medium,ceftriaxone(CTRX) reacts with acriflavine(AF) to form an 1:1 ion association complex,which results in quench of the fluorescence of acriflavine.The measurement wavelength of fluorescence quenching is located at λ_ex/λ_em=266 nm/506 nm.The linear range for CTRX is from 0.056 mg/L to 5.0 mg/L,and its detection limit is 0.016 mg/L.The fluorescence spectra characteristics,the optimum reaction conditions and the effect of additive substances were investigated.The composition of ion-association complexes was discussed also.Based on the ion-association reaction,a high sensitive,simple and rapid method was proposed for the determination of CTRX.The method was applied for the determination of CTRX in human serum and urine samples with satisfactory results.
- ceftriaxone,
- acriflavine,
- fluorescence quenching
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